The B/B Homodimerizer (AP20187) is a synthetic, cell-permeable ligand that can be used to induce homodimerization of fusion proteins containing the DmrB domain. The B/B Homodimerizer has been tested in vitro and in mice. It is nontoxic. We suggest testing various B/B Homodimerizer concentrations within the recommended range (0.01–100 nM for in vitro use or 0.005–10 mg/kg in mice) in order to obtain a complete dose-response profile. The homodimerizer has been widely used to study signal transduction pathways (by inducing oligomerization of cell surface receptor proteins), but it can be used to induce the activity/re-localization of any protein that is affected by oligomerization. The B/B Homodimerizer is identical to the AP20187 ligand, which was previously supplied by ARIAD Pharmaceuticals, Inc.
B/B Washout Ligand—for use with the iDimerize Inducible Homodimer Systems
B/B Washout Ligand is a membrane-permeant ligand that dissociates protein interactions induced by the B/B Homodimerizer (T1/2 of ~10 minutes after adding it to target cells treated with B/B Homodimerizer).
A/C Heterodimerizer (AP21967)—for use with the iDimerize Inducible Heterodimer & Inducible Expression Systems
The A/C Heterodimerizer (AP21967) is a cell permeable ligand used to induce heterodimerization of two different fusion proteins, containing the DmrA and DmrC domains respectively. The A/C Heterodimerizer has been tested in vitro and in mice. It is non-toxic to cells at up to 1 µM concentrations and in mice at up to 30 mg/kg doses. We suggest testing various A/C Heterodimerizer concentrations within the recommended range (0.05 nM–500 nM for in vitro use) in order to obtain a complete dose-response profile. The A/C Heterodimerizer is identical to the AP21967 ligand, which was previously supplied by ARIAD Pharmaceuticals, Inc.
D/D Solubilizer—for use with the iDimerize Reverse Dimerization System
The D/D Solubilizer is a synthetic, cell-permeable ligand that can be used to disrupt oligomerization of fusion proteins containing the DmrD domain. The D/D Homodimerizer has been tested in vitro and in mice for applications including inducible secretion of proteins. It is nontoxic. We suggest testing various D/D Homodimerizer concentrations within the recommended range (10–500 nM) for different lengths of time (30 minutes to 12+ hours) in order to obtain a complete dose-response profile. The D/D Solubilizer performs the same function as the AP21998 ligand, which was previously supplied by ARIAD Pharmaceuticals, Inc. It is a different molecule than AP21998.
