After fusing your protein of interest to one to the dimerization tags provided with iDimerize Homodimer Systems (DmrB) or iDimerize Heterodimer Systems (DmrA and DmrC), you may wish to montitor the protein's expression by SDS-PAGE followed by Western blot. Clontech provides sensitive antibodies that give a clean band on a Western blot. The DmrA and DmrB antibodies are monoclonal mouse antibodies, and the anti-DmrC antibody is polyclonal, derived from rabbit.
The DmrB and DmrA domains are highly homologous so the antibodies cross-react with each others' domains. The anti-DmrC antibody does not cross react with the other domains.
The antibodies are also able to detect fusions created using ARGENT regulation systems:
- DmrA is identical to wild type FKBP12
- DmrB is identical to the F36V mutant of FKBP12
- DmrC is identical to the T82L mutant of FRB