ARGENT Cell Signaling Regulation Kits from ARIAD—Now Available from Clontech
Clontech is the exclusive provider of iDimerize products—homodimerization and heterodimerization systems and ligands (e.g. AP20187, AP21967) which were previously provided by ARIAD under the brand name ARGENT. The tables below show the iDimerize nomenclature and the equivalent ARGENT products and components.
The B/B Homodimerizer (AP20187) induces dimerization of two proteins that each contain the DmrB homodimerization domain (see table below explaining domain nomenclature).
The A/C Heterodimerizer (AP21967) induces dimerization of a protein possessing the DmrA domain and a second protein containing the DmrC domain.
The D/D Solubilizer (alternative to AP21998) induces dissociation/disaggregation of proteins possessing DmrD domains. DmrD causes automatic self-association of proteins fused to it.
The A/A Homodimerizer (AP1510) induces homodimerization of two proteins that each contain the DmrA dimerization domain. The A/A Homodimerizer is provided for existing users to complete ongoing projects. For new homodimerization studies we recommend use of DmrB domains and the B/B Homodimerizer.
ARIAD Ligand Name
Equivalent Clontech System
D/D Solubilizer (A different molecule which is functionally equivalent to AP21998)
The amino acid sequences of the FV and FV' domains are identical; however there are significant differences at the nucleotide sequence level which reduce the potential for recombination between the repeating domain within the pC4M-FV2E plasmid (pHom-Mem1). The same is true of the F and F' domains within pC4M-FV2E (pHet-Mem1). Clontech does not distinguish between the two when referring to the dimerization domain.