Analyzing protein function is a key focus in cell biology research. Clontech’s lentiviral and retroviral ProteoTuner systems make it possible to investigate the function of a specific protein of interest directly—by rapidly changing the abundance of the protein itself.
Protein Regulation Mechanism
These systems utilize a ligand-dependent destabilization domain (DD) modified from the DD of FKBP12 (1), and the ligand Shield1 to reversibly stabilize and destabilize a DD-tagged protein of interest in a predictable and dose-dependent manner. Your protein of interest is fused to a DD tag and rapidly stabilized by adding Shield1 ligand to the culture medium.
N or C Terminal Fusions to Your Protein
Most of the proteins that Clontech has tested show a better destabilization profile when the DD tag is fused to the N-terminus of the protein of interest (Systems N). Specific DD tag mutants for C-terminal tagging are available as well (Systems C); however they have a slightly reduced destabilization activity in the absence of the Shield1 ligand.