ProteoTuner inducible protein stabilization systems utilize ligand-dependent destabilization domains (DD) and the Shield1 ligand to reversibly stabilize and destabilize a DD-tagged protein of interest in a predictable and dose-dependent manner. The destabilization domain of the ProteoTuner Shield System was modified from the DD of FKBP12 (1).
Shield1 stabilizes proteins tagged with a mutated FKBP destabilization domain (DD). It is used to protect DD-tagged proteins from proteasomal degradation. The amount of Shield1 can be varied to tune the amount of your stabilized protein of interest within the cell.