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Products >  Inducible_Systems >  Inducible_Protein_Stabilization >  ProteoTuner_Selection_Guide

Inducible Protein Stabilization Systems Product Selection Guide

The ProteoTuner systems use a powerful, unique technology to regulate the quantity of your protein of interest, at the post translational level. ProteoTuner technology has been successfully used with cytosolic and membrane-bound proteins in a variety of cell types, organisms, and animal models.

Application Recommended Systems Comments
For easy-to-transfect cells

N-terminal DD tag

ProteoTuner Shield System N

ProteoTuner Shield System N (w/AcGFP1)

Most of the proteins that Clontech has tested show a better destabilization profile when the DD tag is fused to the N-terminus of the protein of interest.

Available with or without a built-in green fluorescent protein transfection control.

C-terminal DD tag

ProteoTuner Shield System C

A specific DD tag mutant for C-terminal tagging; provides a slightly reduced destabilization activity in the absence of Shield1.

For difficult-to-transfect, dividing or non-dividing cells

N-terminal DD tag

Lenti-X ProteoTuner Shield System N

Lenti-X ProteoTuner Shield System N (w/ZsGreen1)

Most of the proteins that Clontech has tested show a better destabilization profile when the DD tag is fused to the N-terminus of the protein of interest.

Available with or without a built-in green fluorescent protein transfection control.

C-terminal DD tag Lenti-X ProteoTuner Shield System C A specific DD tag mutant for C-terminal tagging; provides slightly reduced destabilization activity in the absence of Shield1.
For difficult-to-transfect, dividing cells

N-terminal DD tag

Retro-X ProteoTuner Shield System N

Retro-X ProteoTuner Shield System N (w/ZsGreen1)

Most of the proteins that Clontech has tested show a better destabilization profile when the DD tag is fused to the N-terminus of the protein of interest.

Available with or without a built-in green fluorescent protein transfection control.

Shield1 ligand

Controlling DD activity

Shield1

Membrane-permeable small molecule; binds specifically to the DD tag, to inactivate protein degradation.

ProteoTuner Accessories

Confirming DD expression

DD Monoclonal Antibody

Detect DD-N and DD-C tagged proteins.

Selective Co-IP of newly formed protein complexes

ProteoTuner Immunoprecipitation Kit

Choose when, and for how long, to stabilize protein complexes before you immunoprecipitate them.

Control and confirm DD-tagged protein levels

ProteoTuner Quantitation System

Quantitate DD-tagged protein stabilization without a lengthy Western blot protocol

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