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Products >  Inducible_Systems >  Inducible_Protein_Stabilization >  ProteoTuner_Overview

ProteoTuner Technology Overview

  • Introduction and Mechanism Overview
  • Two Core Elements—DD and Shield1
  • Applications

Rapid, Precise Control of Protein Levels

When investigating complicated questions, a streamlined approach often yields the best results. Having the right tools enables you to make the most of every experiment, including those that seek to explore the intricate world of protein function.

Understanding the biological role of a protein is best accomplished by removing it and then studying the consequences: the loss or gain of various cellular functions. There are many well-established methods for removing proteins, including gene knockdown, the use of Cre-Lox technology for gene inactivation, mutations that alter gene function, RNA interference, and various inducible transcription systems. However, all of these approaches target gene function at the DNA and mRNA levels, and most require considerable set-up time.

In contrast, ProteoTuner systems allow quick, predictable regulation of protein presence or absence by acting directly on the protein, using a small, cell-permeable synthetic compound. This post-translational regulation offers a number of benefits, from speed and convenience, to precise tuning and reversible control.

In this system, a protein of interest is tagged with the DD, which is stable in the presence of the Shield1 ligand. Thus, a fusion protein can accumulate and be detected by various assays. Removal of the ligand destabilizes the fusion protein and leads to proteolysis.

Features of ProteoTuner Systems
Features Benefits Notes
Rapid kinetics When Shield1 is added to the medium, the DD-fusion protein can accumulate to detectable levels within 15–20 minutes. Conversely, upon Shield1 removal, the half-time for the protein’s degradation can be as short as 30 minutes. ProteoTuner controls the protein level by acting directly on the post-translated protein, unlike other systems that act either at the transcriptional (inducible gene expression systems) or translational level (RNA interference).
Precise tuning Reduce or increase protein level as needed by decreasing or increasing the concentration of Shield1.
Reversible control Do multiple experiments on the same cells to generate statistically significant data. Easily confirm data. Protein level is ligand-dependent. You can repeat experiments multiple times by simply removing or adding Shield1.
Single-vector system

Make just one fusion construct:

  • Convenient
  • Time-saving
  • Plasmid and viral formats
  • With or without drug resistance
  • With or without fluorescent markers

The ProteoTuner Mechanism

ProteoTuner technology is based on a destabilizing domain (DD) that can confer its instability to a fused protein of interest. This destabilization can be rescued in a reversible and dose-dependent manner with the addition of a small molecule specific to the DD, Shield1. The definitive mechanism of DD regulation is not fully understood, although it is known that cytoplasmic DD degradation is mediated by the ubiquitin-proteasome system (1).

Reference

  1. Egeler, E.L. et al. (2011) J of Biol Chem. 286(36):31328–31336.

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The genetic fusion of the DD to the gene of interest ensures the specificity of the ProteoTuner system, while the small-molecule control via Shield1 confers reversibility and dose-dependence on protein stability and function.

Destabilizing Domain

The destabilizing domain (DD) is mutant L106P of FKBP12 (12 kD), which is rapidly degraded in mammalian cells. The FKBP12 protein itself is well-characterized for its ability to form a tight complex with immunosuppressive drugs, e.g., FK505 and rapamycin (1).

This L106P mutant was one of the most potent destabilizing domains found in the initial screen. When cells expressed a yellow fluorescent protein (YFP) fusion of this mutant, 1–2% of normal YFP levels was seen in the absence of its ligand. The addition of Shield1 stabilized the L106P-YFP fusion in a dose-dependent fashion, and full stability was achieved with 1 µM of the ligand (2).

Shield1 Ligand

The ligand, Shield1, is a small synthetic molecule with a structural bump that binds to the FKBP12 mutant (L106P) with 1600-fold higher affinity than it does to wild-type FKBP12. The molecular size (750 kD) of Shield1 makes it membrane-permeable, allowing it to easily penetrate into different cellular compartments. Based on microarray data that compared the expression profile of genes in NIH3T3 cells treated with varying concentrations of Shield1, the ligand is free of off-target effects on gene expression (3).

Chemical structure of the Shield1 ligand

Chemical structure of the Shield1 ligand.

Reference

  1. Pollock, R. & Clackson, T. (2002) Curr Opin Biotechnol. 13(5):459–467.
  2. Banaszynski, L.A. et al. (2006) Cell 126(5):995–1004.
  3. Maynard-Smith, L.A. et al. (2007) J Biol Chem. 282(34)24866–24872.

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What can you do with ProteoTuner technology?

The control of protein levels enabled by ProteoTuner systems opens up research possibilities in the areas of both protein function and promoter activity.

Use ProteoTuner Systems to study protein function, promoter activity

Areas of study made possible with ProteoTuner Systems.

Protein Function Studies—Loss of Function and/or Gain of Function

  • Remove your protein for long periods of time and study the effects
  • Remove your protein for a short period of time, and then add it back
  • Activate a protein that would otherwise be inactive in given cells or at a given time

Promoter Studies

  • Measure promoter activity based on the amount or intensity of accumulated fluorescence
  • Determine which inducer activates or inactivates your promoter of interest

View the Data

ProteoTuner technology has been used to study the intricacies of a wide range of subjects, including cell signaling, viral replication, cytoskeleton rearrangement, neurodegenerative diseases, and cancer biology. Our tech notes and citation links below provide a detailed look into the capabilities of ProteoTuner systems.

Which ProteoTuner system is suitable for your application?

Visit our selection guide to choose the best system for your experiments.

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