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Products >  Inducible_Systems >  Inducible_Protein_Stabilization >  Plasmid

Protein Regulation with Rapid Kinetics-ProteoTuner Systems

Rapidly regulate the quantity of your protein of interest, at the post translational level. The ProteoTuner systems use a powerful, unique technology to control your protein in living cells. ProteoTuner technology has been successfully used with cytosolic and membrane-bound proteins in a variety of cell types, organisms, and animal models.

One Vector/One Ligand System

  1. A 12 kDa destabilizing domain (DD) that, when fused to a protein of interest, destabilizes the protein by targeting it for proteasomal degradation. Plasmid, retroviral and lentiviral PTuner vectors are available that carry the DD domain for fusion at either the N- or C-terminal of your protein.
  2. A membrane-permeable small molecule (750 Da) ligand, Shield1, which protects the DD fusion protein from being degraded and allows it to accumulate in the cell. Stabilization of the DD-protein occurs in as little as 15–30 minutes.

We also offer the ProteoTuner Tag Kit, which provides you with a ProteoTuner tag sequence so you can quickly and easily convert any Living Colors fluorescent protein-C vector into a ProteoTuner vector. The Kit contains a ProteoTuner destabilization domain (DD) source vector plus a primer mix that lets you PCR-amplify the DD tag sequence. You can then use an In-Fusion Cloning Kit to insert the DD tag sequence upstream of the fluorescent protein coding sequence in any of our fluorescent protein-C vectors. The resulting vector will be able to express a protein of interest that is N-terminally-tagged with a fluorescent protein and subject to ligand-dependent degradation.

At-A-Glance Documents Images & Data Resources

Features

  1. Rapid kinetics: Protein level changes in minutes allows accurate functional analysis
  2. Precise tuning: Precise control of protein level is key to analyzing biological activity
  3. Reversible control: 'Protein on' to 'off' and back makes multiple studies easier
  4. Gets rid of accumulated protein: On demand rapid degradation of accumulated protein is important for analysis of essential proteins

Applications

  • Protein function in pathways
  • Functional analysis of subunits of a protein complex
  • Functional analysis of essential proteins

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
631072 ProteoTuner™ Shield System C Each $700.00 License Statements
632172 ProteoTuner™ Shield System N Each $700.00 License Statements
632168 ProteoTuner™ Shield System N (w/ AcGFP1) Each $700.00 License Statements *
631091 ProteoTuner™ Tag Kit 25 Rxns $600.00 License Statements
632188 Shield1 5 mg $1,900.00 License Statements
632189 Shield1 500 uL $350.00 License Statements
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

 


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