Protein Regulation with Rapid Kinetics-ProteoTuner Systems
Rapidly regulate the quantity of your protein of interest, at the post translational level. The ProteoTuner systems use a powerful, unique technology to control your protein in living cells. ProteoTuner technology has been successfully used with cytosolic and membrane-bound proteins in a variety of cell types, organisms, and animal models.
One Vector/One Ligand System
A 12 kDa destabilizing domain (DD) that, when fused to a protein of interest, destabilizes the protein by targeting it for proteasomal degradation. Plasmid, retroviral and lentiviral PTuner vectors are available that carry the DD domain for fusion at either the N- or C-terminal of your protein.
A membrane-permeable small molecule (750 Da) ligand, Shield1, which protects the DD fusion protein from being degraded and allows it to accumulate in the cell. Stabilization of the DD-protein occurs in as little as 15–30 minutes.
We also offer the ProteoTuner Tag Kit, which provides you with a ProteoTuner tag sequence so you can quickly and easily convert any Living Colors fluorescent protein-C vector into a ProteoTuner vector. The Kit contains a ProteoTuner destabilization domain (DD) source vector plus a primer mix that lets you PCR-amplify the DD tag sequence. You can then use an In-Fusion Cloning Kit to insert the DD tag sequence upstream of the fluorescent protein coding sequence in any of our fluorescent protein-C vectors. The resulting vector will be able to express a protein of interest that is N-terminally-tagged with a fluorescent protein and subject to ligand-dependent degradation.