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Products >  Inducible_Systems >  Inducible_Protein_Stabilization >  Immunoprecipitation

ProteoTuner Immunoprecipitation Kit

Cells often respond to environmental signals by synthesizing new proteins, forming new protein complexes, or post-translationally modifying existing proteins. The ProteoTuner Immunoprecipitation Kit gives you the ability to selectively distinguish these novel events from the existing protein pool, and to better understand cellular responses to external stimuli.

The kit allows you to choose when, and for how long, to stabilize protein complexes before you immunoprecipitate them, so it can be used to increase the signal-to-noise ratio for many types of IP studies, including signal transduction studies.

How the ProteoTuner Immunoprecipitation Kit Works

Clontech’s ProteoTuner Immunoprecipitation Kit is a novel application of ProteoTuner protein stabilization technology, which enables you to clone a protein of interest in frame with a 12 kD destabilization domain (the DD tag). Once your ProteoTuner vector (sold separately) is transfected into cells, your protein of interest is constitutively expressed—but also continually targeted to the proteasome and degraded. However, adding the membrane-permeable small molecule Shield1 to the culture medium protects the DD fusion protein from being degraded, and allows it to accumulate in a dose-dependent manner. The ProteoTuner Immunoprecipitation Kit provides all the materials you need to IP the newly accumulated protein and co-purify its interacting partners.

Magnetic Bead-Based IP Protocol

The kit contains an anti-DD monoclonal antibody (Clone 24A) that has been selected for its ability to immunoprecipitate very efficiently, lysis and wash buffers, and magnetic IgG beads. Simply mix your lysate with the antibody and pull down using these Mag Capture Beads and a magnetic separator such as the Magnetic Stand for Microcentrifuge Tubes (sold separately; Cat. No. 631964).

At-A-Glance Documents Images & Data Resources

Features

  • Efficient immunoprecipitation of newly-stabilized DD-tagged proteins, in order to analyze recent posttranslational modifications or protein complex formation
  • Uses ProteoTuner technology, and a DD monoclonal antibody optimized for IP (Clone 24A)
  • Magnetic bead-based IP protocol
  • Kit includes anti-DD antibody, anti-IgG-coated magnetic beads, and a buffer set

Applications

  • Understanding cellular responses to external stimuli at the protein level
  • Immunoprecipitation of newly synthesized proteins
  • Selective co-immunoprecipitation of newly formed protein complexes

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price # of Units Select
635070 ProteoTuner™ Immunoprecipitation Kit 25 Rxns $513.00
 

 

Required Products
Cat. # Product Package Size Price # of Units Select
631964 Magnetic Stand 2 x 1.5 mL $42.00
 

 

Recommended Products
Cat. # Product Package Size Price License # of Units Select
632168 ProteoTuner™ Shield System N (w/ AcGFP1) Each $700.00 License Statements *
632172 ProteoTuner™ Shield System N Each $700.00 License Statements
632173 Lenti-X™ ProteoTuner™ Shield System N Each $740.00 License Statements
632189 Shield1 500 uL $350.00 License Statements
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 
 


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