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Products >  Glycobiology >  Glycobiology_Enzymes_and_Reagents >  alpha1_3-4_L_Fucosidase

Glycobiology Enzymes: Alpha 1, 3/4-L Fucosidase

Alpha 1, 3/4-L Fucosidase (systemic name: Alpha-L-Fucoside Fucohydrolase) is a useful glycobiology enzyme and specifically cleaves alpha 1,3 and alpha 1,4 fucoside bonds, liberating L-fucose from lacto-N-fucopentaose II, lacto-N-fucopentaose III and N-acetyllactosamine type tri-antennary and tetra-antennary sugar chains containing alpha 1,3 fucoside bonds. It also liberates L-fucose from alpha 1-acid glycoproteins.
  At-A-Glance   Documents   Images & Data

Applications

  • Specifically cleaves alpha1,3 and alpha1,4 fucoside bonds
  • Liberates L-fucose from lacto-N-fucopentaose II, lacto-N-fucopentaose III and N-acetyllactosamine type tri-antennary and tetra-antennary sugar chain with alpha1,3 fucoside bonds
  • Also liberates L-fucose from alpha1-acid glycoprotein

Source

Streptomyces sp. 142

Storage

Store at –20°C. The stock solution is stable for at least 12 months at 4°C. Avoid repeated freeze-thaw cycles.

Properties

  • Systematic name: Alpha-L-Fucoside fucohydrolase (E.C. 3.2.1.51)
  • Molecular weight: 40 kDa
  • Concentration: 1 mU/mL
  • Km (substrate):
    • Substrate 1: 10.1 µM (PA-lacto-N-fucopentaose II)
    • Substrate 2: 1.08 µM (PA-lacto-N-fucopentaose III)
  • Optimum pH: pH 6.0 (Substrate 2)
  • Range of pH stability: pH 4.5–7.0 (37°C, 60 min.)
  • Stabilizer: 0.1% NP-40, 0.1% Brij-58, and 0.02% BSA

Form

In a solution of 50 mM potassium phosphate buffer (pH 6.0) containing 0.1 M NaCl, 0.02% NaN3 and 0.1% Brij-58

References

  1. Sano, M. et al. (1992) J. Biol. Chem. 267:1522-1527.

Definition of Activity

One unit is defined as the amount of enzyme required to hydrolyze 1 µmol of pyridylamino-lacto-N-fucopentaose III in 1 minute at 37°C and pH 6.0.
 
 
Products
Cat. # Product Contents Size Price Units Select
4453 Alpha 1, 3/4-L-Fucosidase 100 uU $372.00
 

 

 
 

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