Lacto-N-Biosidase may be used during glycoprotein analysis to better study the structure and function of glycoprotein and glycolipid sugar chains. This product can be used for specific oligosaccharide hydrolysis of type-I chain oligosaccharides, producing lacto-N-biose (Gal β 1-3 GlcNAc) as a byproduct, but not oligosaccharide hydrolysis of type-II chain oligosaccharides. As a result, Lacto-N-Biosidase can be used to distinguish type-I versus type-II glycoprotein as well as glycolipid sugar chains. When used in conjunction with α -1,3/4-Fucosidase, Lacto-N-Biosidase can also help distinguish Sialyl-Lewis x and Sialyl-Lewis a structures.
One vial of Lacto-N-Biosidase (100 µU) allows 50–100 enzymatic digestions of up to 10 pmol of sugar chains.