Glycopeptidase F, also known as PNGase F or Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase, removes N-glycan groups from glycoproteins and glycopeptides. Deglycosylation enables proteomic analysis, including MALDI-TOF mass spectrometry. PNGase F is often used to determine if proteins contain glycosylated peptides and if deglycosylation affects a protein's enzymatic activity. Removal of N-linked glycans prior to MALDI-TOF and other mass spectrometry procedures may also be performed to reduce protein heterogeneity and thereby facilitate proteomic analysis. PNGase F is one of the most widely used enzymes for deglycosylation of glycoproteins and glyopeptides.