Recombinant Endoglycoceramidase II (EGCase II) with Activator II (ACT II) is an endo-type enzyme that cleaves the linkages between oligosaccharides and ceramides in acidic and neutral glycosphingolipids, producing intact oligosaccharide and ceramide byproducts. Using EGCase II with protein activator Act II permits cell surface glycosphingolipid degradation in living cells without damage to other cell components.
This enzyme does not hydrolyze cerebroside, sulfatide, sphingomyelin, glycoglycerolipid, glycoprotein and galactosylceramide linkages of gala-type glycosphingolipids. Cell-surface globo-type glycosphingolipids are strongly resistant to hydrolysis by EGCase II ACT RE. EGCase II ACT RE is a mixture of highly purified recombinant EGCase II and Act II.