Deliver Cre recombinase protein to mammalian cells in the absence of nucleic acids (i.e., no plasmid or virus is required), rapidly and efficiently. These gesicles are cell-derived nanovesicles containing concentrated Cre recombinase protein that, when applied to your target cells, fuse with the plasma membrane and discharge into the lumen of the cell. The enzyme is transported to the nucleus via its nuclear localization signal where it can carry out Cre-mediated recombination between loxP sites. Since there is no coding gene present, there is no risk of sustained Cre recombinase expression in your target cells limiting the chance of unintended recombination events.
What is Cre Recombinase?
Cre recombinase is a site-specific recombinase isolated from P1 bacteriophage that catalyzes the recombination between two 34 bp loxP sites. The enzyme is widely used for knock-out and knock-in studies: any DNA sequence located between two tandem repeats of loxP can be excised to create a deletion mutant, or a plasmid containing a single loxP site can undergo site-specific integration into a genome containing loxP to create an insertion mutant).
What are the Benefits of Gesicle Technology?
Far simpler to use than either plasmid or viral gene delivery. Simply apply 10–20 µl of the gesicles to your target cells; no preparation or pre-treatment is required.
Can be used with many cell typesany, including dividing and non-dividing cells, primary cells, and cell lines.
Ensure efficient delivery and high activity of Cre recombinase (see Images and Data tab). When using gesicle delivery there is no delay for transcription/translation: the protein is present and active as soon as you apply the gesicles to your cells.
Since there is no coding gene present, there is no risk of sustained Cre recombinase expression in your target cells. It is present when you need it and gone when you don’t.