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Genome Editing


Products >  Genome_Editing >  Cre_Recombinase >  AAV2-Cre_Recombinase

Helper Virus-Free Preparation of AAV Particles to Deliver Cre Recombinase

The Cre-lox system is used to introduce deletions, insertions, translocations, and inversions at a specific genomic locus. The system utilizes Cre recombinase, a bacteriophage P1 enzyme that recognizes a short target DNA sequence (loxP site). The AAVpro Helper Free System AAV2-CRE Recombinase is a kit for the preparation of serotype 2 adeno-associated virus (AAV2) viral particles to deliver the Cre recombinase gene cassette to mammalian cells. High-titer AAV2 particles can be prepared without a helper virus. HEK 293T cells are transfected by plasmids encoded in this kit, and AAV Extraction Solution is used for efficient AAV2 particle isolation.

  At-A-Glance   Documents   Images & Data

Features

  • Helper virus-free preparation of high-titer AAV2 particles encoding the Cre recombinase gene under the control of the CMV promoter
  • Enables delivery of the Cre recombinase gene to hard-to-transfect mammalian cells, including proliferating and non-proliferating cells
  • The Cre recombinase protein mediates recombination between loxP sites, allowing gene inversion, translocation, or deletion

Applications

  • Genome editing—transduction of target cells containing a floxed DNA sequence with the AAV2-CRE Recombinase viral particles results in recombination between the loxP sites
  • Generating transgenic mice

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
6652 AAVpro® Helper Free System (AAV2-CRE Recombinase) 20 ug each $894.00 License Statements
 

 

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