The Lenti-X CRISPR/Cas9 System and Lenti-X Tet-On 3G CRISPR/Cas9 System are complete systems for lentiviral-mediated CRISPR/Cas9 genome editing. By using lentivirus to deliver sgRNA and Cas9, these systems allow you to achieve targeted genome editing in cell lines that are difficult to transfect. Each system includes Lenti-X Packaging Single Shots for generating high titers of virus and plasmid sets designed to express Cas9 and your custom sgRNA in target cells. The plasmid for sgRNA expression, pLVX-hyg-sgRNA1, comes prelinearized for easy insertion of your sgRNA sequence, and is supplied in sufficient quantity for construction of 10 different plasmids, each with its own sgRNA sequence.
Whereas the Lenti-X CRISPR/Cas9 System involves constitutive expression of Cas9 in target cells, the Lenti-X Tet-On 3G CRISPR/Cas9 System incorporates the Tet-On 3G transactivator protein, which enables users to induce Cas9 expression via the addition of doxycycline. By allowing for tight control of Cas9 expression, the Lenti-X Tet-On 3G CRISPR/Cas9 System enables users to minimize the likelihood of toxicity and off-target effects associated with persistent Cas9 expression in cell culture.