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Products >  Genome_Editing >  CRISPR_Cas9 >  lentiviral_systems

Lenti-X CRISPR/Cas9 Systems

The Lenti-X CRISPR/Cas9 System and Lenti-X Tet-On 3G CRISPR/Cas9 System are complete systems for lentiviral-mediated CRISPR/Cas9 genome editing. By using lentivirus to deliver sgRNA and Cas9, these systems allow you to achieve targeted genome editing in cell lines that are difficult to transfect. Each system includes Lenti-X Packaging Single Shots for generating high titers of virus and plasmid sets designed to express Cas9 and your custom sgRNA in target cells. The plasmid for sgRNA expression, pLVX-hyg-sgRNA1, comes prelinearized for easy insertion of your sgRNA sequence, and is supplied in sufficient quantity for construction of 10 different plasmids, each with its own sgRNA sequence.

Whereas the Lenti-X CRISPR/Cas9 System involves constitutive expression of Cas9 in target cells, the Lenti-X Tet-On 3G CRISPR/Cas9 System incorporates the Tet-On 3G transactivator protein, which enables users to induce Cas9 expression via the addition of doxycycline. By allowing for tight control of Cas9 expression, the Lenti-X Tet-On 3G CRISPR/Cas9 System enables users to minimize the likelihood of toxicity and off-target effects associated with persistent Cas9 expression in cell culture.

  At-A-Glance   Documents   Images & Data

Features

  • Viral delivery of the Cas9 gene and an sgRNA allows for genome editing in hard-to-transfect mammalian cells, including proliferating and non-proliferating cells
  • Lenti-X Packaging Single Shots make generating high-titer lentivirus extremely simple
  • Separate systems available for either constitutive or inducible Cas9 expression in target cells
  • Tightest possible control of Cas9 expression (Lenti-X Tet-On 3G CRISPR/Cas9 System) allows you to minimize the likelihood of toxicity and off-target effects associated with persistent Cas9 expression in cell culture

Applications

  • Lentivirus-based delivery of a user-defined sgRNA and Cas9 for mammalian genome editing using CRISPR/Cas9 technology

Lenti-X CRISPR/Cas9 System (Cat. # 632629)

  • pLVX-hyg-sgRNA1 Vector (Linear)
  • pLVX-puro-Cas9 Vector
  • Stellar Competent Cells
  • Guide-it Ligation Components v2
  • Lenti-X Packaging Single Shots

Lenti-X Tet-On 3G CRISPR/Cas9 System (Cat. # 632633)

  • pLVX-hyg-sgRNA1 Vector (Linear)
  • pLVX-TRE3G-Cas9-puro Vector Set
  • pLVX-EF1a-Tet3G Vector
  • Stellar Competent Cells
  • Guide-it Ligation Components v2
  • Lenti-X Packaging Single Shots

pLVX-hyg-sgRNA1 Vector System (Cat. # 632630)

  • pLVX-hyg-sgRNA1 Vector (Linear)
  • Guide-it Ligation Components v2
  • Stellar Competent Cells

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Components List to determine kit components. Certificates of Analysis and Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price License Units Select
632629 Lenti-X™ CRISPR/Cas9 System 1 System $1,096.00 License Statements
632630 pLVX-hyg-sgRNA1 Vector System 10 Rxns $325.00 License Statements
632631 pLVX-puro-Cas9 Vector 20 uL $626.00 License Statements
632633 Lenti-X™ Tet-On® 3G CRISPR/Cas9 System 1 System $1,768.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

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