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Genome Editing


Products >  Genome_Editing >  CRISPR_Cas9 >  RNA_Transfection

Xfect RNA Transfection Reagent for CRISPR/Cas9-mediated gene editing

Xfect RNA Transfection Reagent efficiently delivers mRNA coding for Cas9 and single guide RNA (sgRNA) directed against your target gene to a wide variety of mammalian cells for CRISPR/Cas9-mediated gene editing. Transfection with RNA precludes genomic integration that often follows transfection with plasmid DNA. While most transfection reagents can be harmful to your cells, the Xfect RNA Transfection Reagent has very low cytotoxicity. This reagent creates biodegradable nanoparticles when combined with RNA, enabling efficient transfection of mammalian cells without any damaging cytotoxic effects.

The Xfect RNA Transfection Reagent can also be used for transfection with microRNA and siRNA. The protocol is simple, and transfections can be carried out entirely in the presence of serum.

When used in conjunction with the Xfect Transfection Reagent, the Xfect RNA Transfection Reagent can be used for efficient cotransfection of mammalian cells with both DNA and RNA.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Single reagent for transfection with messenger RNA (mRNA), guide RNA (sgRNA), microRNA (miRNA), and siRNA
  • High transfection efficiency and very low cytotoxicity in cell lines and primary cells
  • Simple, serum-compatible protocol
  • Complete system that includes all components necessary for transfection

Applications

  • CRISPR/Cas9
  • RNA transfection
  • Transient transfection without genomic integration

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
631450 Xfect™ RNA Transfection Reagent 1.2 mL $218.00 License Statements
 

 

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