Xfect RNA Transfection Reagent efficiently delivers mRNA coding for Cas9 and single guide RNA (sgRNA) directed against your target gene to a wide variety of mammalian cells for CRISPR/Cas9-mediated gene editing. Transfection with RNA precludes genomic integration that often follows transfection with plasmid DNA. While most transfection reagents can be harmful to your cells, the Xfect RNA Transfection Reagent has very low cytotoxicity. This reagent creates biodegradable nanoparticles when combined with RNA, enabling efficient transfection of mammalian cells without any damaging cytotoxic effects.
The Xfect RNA Transfection Reagent can also be used for transfection with microRNA and siRNA. The protocol is simple, and transfections can be carried out entirely in the presence of serum.
When used in conjunction with the Xfect Transfection Reagent, the Xfect RNA Transfection Reagent can be used for efficient cotransfection of mammalian cells with both DNA and RNA.