Features- A single vector expresses your target-specific sgRNA from the human U6 promoter, and Cas9 nuclease and a bright fluorescent marker (either ZsGreen1 or tdTomato) are expressed from a bidirectional CMV promoter
- tdTomato and ZsGreen1 are exceptionally bright fluorescent proteins, 6x and 2.5x brighter than EGFP, respectively; these markers can be used to monitor transfection efficiency and/or to enrich/isolate transfected cells by flow cytometry
- Simple plasmid construction—design oligos specific to your target sequence, and clone into the pre-linearized plasmid using the included ligation reagents and high-efficiency competent cells
- Includes sufficient reagents for construction of 10 different target (sgRNA) expression plasmids
Applications- Cloning and expression of a target single guide RNA (sgRNA) for mammalian genome editing using CRISPR/Cas9 technology
ComponentsGuide-it CRISPR/Cas9 System (Green) (Cat. No. 632601) - pGuide-it-ZsGreen1 Vector (Linear)
- Guide-it Ligation Components
- Stellar Competent Cells
Guide-it CRISPR/Cas9 System (Red) (Cat. No. 632602) - pGuide-it-tdTomato Vector (Linear)
- Guide-it Ligation Components
- Stellar Competent Cells
Additional InformationPlease see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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