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Products >  Genome_Editing >  CRISPR_Cas9 >  Legacy_sgRNA_In_Vitro_Transcription_and_Screening

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Legacy In Vitro Transcription and Screening Kits for sgRNA

Legacy kits for in vitro production and testing of sgRNA (single guide RNA) for Cas9/CRISPR studies

Components

Guide-it sgRNA In Vitro Transcription Kit (Cat. No. 631438)

  • 1 each Guide-it sgRNA In Vitro Transcription Components (Cat. No. 631441; 10 rxns; Not sold separately)
    • 50 µl Guide-it Scaffold Template (20 ng/µl)
    • 70 µl Guide-it In Vitro Transcription Buffer
    • 30 µl Guide-it T7 Polymerase Mix (33 U/µl)
    • 500 µl RNase Free Water
  • 1 each NucleoSpin Gel and PCR Clean-Up (Sample) (Cat. No. 740609.10; 10 preps; Not sold separately)
  • 1 each High Yield PCR EcoDry Premix (Cat. No. 639278; 24 rxns)
Guide-it sgRNAScreening Kit (Cat. No. 631440)
  • 1 each Guide-it Recombinant Cas9 Nuclease (Cat. No. 631442; 30 rxns; Not sold separately)
    • 30 µl Guide-it Recombinant Cas9 Nuclease (500 ng/µl)
    • 30 µl 10X Cas9 Reaction Buffer
    • 30 µl 10X BSA
    • 500 µl RNase Free Water
    • 10 µl 2 kb Control Fragment (40 ng/µl)
    • 6 µl Control sgRNA (20 ng/µl)
  • 1 each NucleoSpin Gel and PCR Clean-Up (Sample) (Cat. No. 740609.10; 10rxns; Not sold separately)
  • 1 each High Yield PCR EcoDry Premix (Sample) (Cat. No. 639279; 8 rxns)

The Guide-it Complete sgRNA Screening System (Cat. No. 631439) is a combination of both kits above.

The products listed on this page are being phased out, the replacement products are already available and are described in detail on this page. The new versions of these kits include a greater number of reactions per kit, produce greater yields of RNA, and have simpler protocols that include the use of a high-quality RNA purification kit.

The Guide-it sgRNA In Vitro Transcription Kit (Cat. No. 631438) is used to produce in vitro-transcribed single guide RNAs (sgRNAs) for Cas9/CRISPR studies. The Guide-it sgRNA Screening Kit (Cat. No. 631440) enables you to test the efficacy of different single guide RNAs (sgRNAs) in vitro prior to using them in your target cells. The Guide-it Complete sgRNA Screening System (Cat.No. 631439) is a combination of both kits described above and is used to synthesize and test the efficacy of single guide RNAs (sgRNAs).

Features

  • The products listed on this page are being phased out; the replacement products are described in detail on this page.
  • Three kits for simple evaluation of the efficacy of sgRNA (single guide RNA) for Cas9-mediated gene editing studies
  • In vitro transcribe >4µg of sgRNA per reaction in the Guide-it sgRNA In Vitro Transcription Kit (Cat. No. 631438) for transfection or in vitro screening
  • High-quality recombinant Cas9 protein included in the Guide-it sgRNA Screening kit (Cat. No. 631440)
  • Best value; the Guide-it Complete sgRNA Screening System (Cat. No. 631439) is a combination of both kits allowing you to both produce sgRNA and test its ability to cleave its putative target in vitro
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