Features- Streamlined method for characterizing the variety of indels introduced by gene editing technologies
- Ultrafast protocol includes Terra PCR Direct Polymerase for PCR amplification directly from cells, and the In-Fusion cloning system for ligation-free cloning in 15 minutes
- Ideal for identifying indels caused by Cas9/CRISPR, TALENs, or zinc-finger nucleases based gene editing in a cell population
- Complete kit contains all of the components needed to amplify, clone, and prepare modified target sites for DNA sequence analysis
Applications- Detecting insertions and deletions introduced in mammalian genomic DNA
- Characterizing the variety of indels that are present in a cell population after nuclease-based gene editing
Components- Guide-it Indel Identification Components
- 110 µl Terra PCR Direct Polymerase Mix
- 3 x 1 ml 2X Terra PCR Direct Buffer (with Mg2+, dNTP)
- 400 µl Extraction Buffer 1
- 40 µl Extraction Buffer 2
- 10 µl pUC19 Cloning Vector, linearized (50 ng/µl)
- 200 µl Colony PCR Forward Primer (15 µM)
- 200 µl Colony PCR Reverse Primer (15 µM)
- 6 x 1 ml PCR-Grade Water
- In-Fusion HD Cloning Kit
- NucleoSpin Gel and PCR Clean-Up Kit
- Stellar Competent Cells
Additional InformationPlease see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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