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Products >  Genome_Editing >  CRISPR_Cas9 >  Genotype_Confirmation_Kit

Guide-it Genotype Confirmation Kit

Every cell has two alleles of most genes. CRISPR/Cas9 gene editing can introduce insertion or deletion mutations (indels) on either one or both alleles of a given gene. The Guide-it Genotype Confirmation Kit provides a simple method to determine if a given clone has mutations in one allele (monoallelic), both alleles (biallelic), or is unchanged (wild type). The protocol involves amplification of the target site from cells treated with Cas9 and a single guide RNA (sgRNA). The resulting amplicon is used in an in vitro cleavage reaction with recombinant Cas9 and the same sgRNA. The genotype can be determined by resolving the cleavage products on an agarose gel.

Using this kit after gene editing expedites the process of screening a large number of clones for those with the desired genotype. Each kit contains sufficient reagents for 100 extraction, amplification, and cleavage reactions.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • In vitro cleavage assay with Cas9 and sgRNA allows for identification of monoallelic and biallelic indels
  • Includes highly purified recombinant Cas9 nuclease for in vitro cleavage reaction
  • Streamlined protocol that uses direct amplification of target genomic DNA from cells

Applications

  • CRISPR/Cas9 gene editing
  • Identification of monoallelic and biallelic mutations after CRISPR/Cas9 gene editing

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
632611 Guide-it™ Genotype Confirmation Kit 100 Rxns $428.00 License Statements
 

 

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