Takara Bio offers both monoclonal and polyclonal antibodies raised against recombinant Cas9 protein from Streptococcus pyogenes.
The Guide-it Cas9 Monoclonal Antibody (Clone TG8C1) is a mouse antibody produced by hybridoma cells against full-length recombinant Cas9 protein from Streptococcus pyogenes. This antibody can recognize less than 1 ng of wild-type Cas9 in bacterial and mammalian cell lysates by Western blot.
The Guide-it Cas9 Polyclonal Antibody is a rabbit antibody raised against recombinant Cas9 protein from Streptococcus pyogenes. This antibody recognizes three variants of the Cas9 nuclease: wild-type Cas9, the Cas9 nickase, and Cas9 nuclease-deficient mutants. The antibody is specific and sensitive; no background expression is detected in most mammalian cell lines and the antibody can recognize as little as 0.5 ng of recombinant Cas9 by Western blot.