The Pathway Profiling Systems provide a means to assess signal transduction pathway activation in vivo. These systems consist of sets of vectors that each contain a distinct cis-acting enhancer element upstream of a reporter gene. A single transfection experiment provides a profile of the transcription factors that are affected by a given stimulus, drug candidate, or gene product of interest.
Profile Multiple Signal Transduction Pathways
Most of the cis-acting enhancer elements included in our transcription factor systems are regulated by more than one transcription factor, so multiple signal transduction pathways can be monitored. Reporter expression indicates that a transactivator present in the cell has bound the enhancer element in one of the TransFactor Vectors, indicating that a particular signaling pathway or pathways has been activated.
- Use the Pathway Profiling SEAP System for broad-spectrum profiling.
- Use the Pathway Profiling Luciferase System 4 to study checkpoints in the cell cycle.
Two Reporters Available—Luciferase & SEAP
- Luciferase induction can be measured from cell lysates using a standard assay.
- SEAP can be measured simply by collecting samples of the culture medium without cell lysis. Clontech offers the Great EscAPe SEAP Chemiluminescence Kit 2.0 to measure SEAP activity.