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Fluorescent Proteins & Reporters


Products >  Fluorescent_Proteins_and_Reporters >  Signal_Transduction >  Pathway_Profiles

Cell Signaling Pathway Profiling Systems

The Pathway Profiling Systems provide a means to assess cell signaling pathway activation in vivo. These systems consist of sets of vectors that each contain a distinct cis-acting enhancer element upstream of a reporter gene. A single transfection experiment provides a profile of the transcription factors that are affected by a given stimulus, drug candidate, or gene product of interest.

Profile Multiple Signal Transduction Pathways

Most of the cis-acting enhancer elements included in our transcription factor systems are regulated by more than one transcription factor, so multiple signal transduction pathways can be monitored. Reporter expression indicates that a transactivator present in the cell has bound the enhancer element in one of the TransFactor Vectors, indicating that a particular signaling pathway or pathways has been activated.

  • Use the Pathway Profiling SEAP System for broad-spectrum profiling.
  • Use the Pathway Profiling Luciferase System 4 to study checkpoints in the cell cycle.

Two Reporters Available—Luciferase & SEAP

  • Luciferase induction can be measured from cell lysates using a standard assay.
  • SEAP can be measured simply by collecting samples of the culture medium without cell lysis. Clontech offers the Great EscAPe SEAP Chemiluminescence Kit 2.0 to measure SEAP activity.

At-A-Glance Documents Images & Data

Features

  • Extensive coverage of cell signaling pathways
  • Easy to use & reliable

Applications

  • Study activation of cis-acting enhancer elements in vivo
  • Measure transcription factor activation
  • Quantify differences in activation

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price # of Units Select
631910 Pathway Profiling SEAP System Each $584.00
631907 pAP1(PMA)-SEAP Vector 20 ug $429.00
631906 pAP1(PMA)-TA-Luc Vector 20 ug $429.00
631903 pAP1-SEAP Vector 20 ug $429.00
631901 pSRE-SEAP Vector 20 ug $429.00
631909 pTAL-Luc Vector 20 ug $429.00
631908 pTAL-SEAP Vector 20 ug $429.00
 

 

 


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