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Products >  Fluorescent_Proteins_and_Reporters >  Fluorescent_Proteins_by_Name >  mCherry_Fluorescent_Protein

mCherry Fluorescent Protein

mCherry is a red monomer which matures extremely rapidly, making it possible to see results very soon after activating transcription. It is highly photostable and resistant to photobleaching (1). mCherry has been cited in approximately 200 publications to date.

Developing mCherry

Sequence mutations were introduced to mRFP by the Tsien lab (through directed evolution) in order to produce the final optimized mCherry fluorescent protein.

mCherry Vectors

We have developed mCherry vectors for bacterial expression, fusions, and promoter studies. Vectors are available with lentiviral, retroviral or plasmid delivery. A lentiviral IRES mCherry vector is also available. mCherry sequence files can be found in the Documents tab below.

Anti-mCherry Antibody

mCherry fluorescent protein can be detected with the mCherry Monoclonal Antibody and the DsRed Polyclonal Antibody. Visit the anti-mCherry Antibody page.

CherryPicker Cell Capture Systems

CherryPicker Cell Capture & Separation Systems use a membrane-targeted version of mCherry to identify and capture your cells of interest, without the time or expense of a FACS sorter. These systems allow you to enrich a population of cells that express your protein of interest or that have your active promoter.

Photoactivatable mCherry Fluorescent Protein

PAmCherry is a photoactivatable mutant of mCherry that is non-fluorescent until it is exposed to 350–400 nm light. By selecting which cells or cellular regions to activate, you can track cells, organelles, or proteins of interest against a dark background. You can visualize and monitor photoactivated PAmCherry with the same filter sets used for other red fluorescent proteins, such as DsRed variants and mCherry.

At-A-Glance Documents Images & Data Resources

Features

  • Rapid maturation (t0.5=15 min)
  • Monomeric structure
  • mCherry excitation maximum: 587 nm
    Emission maximum: 610 nm
  • PAmCherry excitation maximum: 575 nm
    Emission maximum: 601 nm
    PAmCherry is non-fluorescent until activated by a short exposure to 350–400 nm light

Applications

mCherry vectors are available for a variety of uses including:

  • Fusions
  • Promoter studies
  • FRET and other quantitative imaging techniques
  • IRES coexpression of mCherry and another protein of interest
  • Enriching a population of cells that express your protein of interest or that have your active promoter with the CherryPicker Cell Capture & Separation Systems
  • Tracking sub-populations of cells, organelles, or protein molecules and visualizing protein dynamics in real time with photoactivatable PAmCherry vectors

References

  1. Shaner, N. C. et al. (2004) Nature Biotechnol. 22(12):1567–1572. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
Products
Cat. # Product Package Size Price License # of Units Select
632522 pmCherry Vector 20 ug $516.00 License Statements *
632523 pmCherry-N1 Vector 20 ug $516.00 License Statements *
632524 pmCherry-C1 Vector 20 ug $516.00 License Statements *
632525 pmCherry-1 Vector 20 ug $516.00 License Statements *
632542 pmR-mCherry Vector 20 ug $557.00 License Statements *
631237 pLVX-IRES-mCherry Vector 20 ug $676.00 License Statements *
632561 pLVX-mCherry-C1 Vector 10 ug $676.00 License Statements *
632562 pLVX-mCherry-N1 Vector 10 ug $676.00 License Statements *
632567 pRetroQ-mCherry-C1 Vector 10 ug $676.00 License Statements *
632568 pRetroQ-mCherry-N1 Vector 10 ug $676.00 License Statements *
631969 pEF1alpha-mCherry-N1 Vector 10 ug $541.00 License Statements *
631972 pEF1alpha-mCherry-C1 Vector 10 ug $541.00 License Statements *
631985 pLVX-EF1alpha-mCherry-C1 Vector 10 ug $676.00 License Statements *
631986 pLVX-EF1alpha-mCherry-N1 Vector 10 ug $676.00 License Statements *
631987 pLVX-EF1alpha-IRES-mCherry Vector 10 ug $676.00 License Statements *
632584 pPAmCherry-N1 Vector 10 ug $574.00 License Statements *
632585 pPAmCherry-C1 Vector 10 ug $574.00 License Statements *
632586 pLVX-PAmCherry-N1 Vector 10 ug $676.00 License Statements *
632587 pLVX-PAmCherry-C1 Vector 10 ug $676.00 License Statements *
632588 pPAmCherry-Mem Vector 10 ug $574.00 License Statements *
632589 pPAmCherry-Actin Vector 10 ug $574.00 License Statements *
632590 pPAmCherry-Tubulin Vector 10 ug $574.00 License Statements *
632591 pPAmCherry-Mito Vector 10 ug $574.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

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* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

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