mCherry is a red monomer which matures extremely rapidly, making it possible to see results very soon after activating transcription. It is highly photostable and resistant to photobleaching (1). mCherry has been cited in approximately 200 publications to date.
Sequence mutations were introduced to mRFP by the Tsien lab (through directed evolution) in order to produce the final optimized mCherry fluorescent protein.
We have developed mCherry vectors for bacterial expression, fusions, and promoter studies. Vectors are available with lentiviral, retroviral or plasmid delivery. A lentiviral IRES mCherry vector is also available. mCherry sequence files can be found in the Documents tab below.
CherryPicker Cell Capture & Separation Systems use a membrane-targeted version of mCherry to identify and capture your cells of interest, without the time or expense of a FACS sorter. These systems allow you to enrich a population of cells that express your protein of interest or that have your active promoter.
Photoactivatable mCherry Fluorescent Protein
PAmCherry is a photoactivatable mutant of mCherry that is non-fluorescent until it is exposed to 350–400 nm light. By selecting which cells or cellular regions to activate, you can track cells, organelles, or proteins of interest against a dark background. You can visualize and monitor photoactivated PAmCherry with the same filter sets used for other red fluorescent proteins, such as DsRed variants and mCherry.