A Better Alternative to EGFP
The AcGFP1 and EGFP sequences have 94% homology at the amino acid level. However, AcGFP1 is a superior alternative for fusion applications because it is a monomer. In contrast, the Aequorea victoria GFPs, AvGFP and EGFP, have been reported to form dimers (1–2).
The monomeric nature of the AcGFP1 fluorescent protein has been confirmed by FPLC gel filtration chromatography, sucrose density gradient ultracentrifugation, and pseudonative gel electrophoresis (please see the Images & Data tab for details).
We offer four antibodies that detect AcGPF1. Visit the anti-AcGFP1 Antibody page.