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Fluorescent Proteins & Reporters


Products >  Fluorescent_Proteins_and_Reporters >  Fluorescent_Proteins >  Transfection_Controls

Fluorescent Labeling & Transfection Control

The Living Colors Constitutive Fluorescent Protein Expression Vectors can be used as transfection markers and to label living cells. They do not contain an MCS. In these vectors, the fluorescent protein is constitutively expressed and can be detected by fluorescence microscopy or flow cytometry, providing direct visual evidence of transfection (or cotransfection) or the ability to monitor cells after transplantation experiments in vivo. Transfected cells can also be used in assays that require fluorescent labeling of whole cells—e.g., cell migration assays. If required, stable transfectants can be selected with neomycin (G418).

Fluorescent Markers

DsRed-Express & DsRed-Express2 are rapidly-maturing variants of Discosoma sp. red fluorescent protein. DsRed-Express2, which retains the fast maturation and high photostability characteristic of the original DsRed-Express, and has been engineered for increased solubility. DsRed-Express2 is particularly suited for stem cell applications and use in other sensitive cell types (1, 2).

E2-Crimson is a far-red derivative of DsRed-Express2. E2-Crimson is extremely bright, very soluble, and matures extremely fast (half time of 26 minutes at 37°C). It exhibits low cytotoxicity and is well-suited for in vivo applications involving sensitive cells such as primary cells and stem cells.

tdTomato is the tandem version of the dimeric red fluorescent protein, Tomato. tdTomato is an extremely bright fluorescent protein and is extremely suitable for in vivo imaging applications.

CMV Promoter Vectors (pCMV)

The immediate early promoter of cytomegalovirus (CMV) is a constitutive promoter with strong transcriptional activity in a variety of cell types.

EF-1 Alpha Promoter Vectors (pEF1a)

Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter that can be used in conditions where other promoters (such as CMV) have diminished activity or have been silenced (as in embryonic stem cells).

At-A-Glance Documents Images & Data Resources

Features

  • Bright fluorescent proteins for labeling
  • Choice of CMV or EF1-a promoters
  • Multiple color options

Applications

  • Basic fluorescent reporter for mammalian gene expression
  • Whole-cell labeling
  • Monitoring transfection efficiencies

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
Products
Cat. # Product Package Size Price License # of Units Select
632539 pCMV DsRed-Express2 Vector 20 ug $557.00 License Statements *
632416 pCMV-DsRed-Express Vector 20 ug $574.00 License Statements *
632556 pCMV-E2-Crimson Vector 20 ug $574.00 License Statements *
632534 pCMV-tdTomato Vector 20 ug $516.00 License Statements *
631979 pEF1alpha-DsRed-Express2 Vector 10 ug $541.00 License Statements *
631981 pEF1alpha-E2-Crimson Vector 10 ug $541.00 License Statements *
631975 pEF1alpha-tdTomato Vector 10 ug $541.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 


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