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Fluorescent Proteins & Reporters


Products >  Fluorescent_Proteins_and_Reporters >  Fluorescent_Proteins >  Subcellular_Labeling

Fluorescent Proteins for Organelle and Subcellular Labeling

Visualize subcellular structures directly and noninvasively by fluorescence microscopy. With these vectors, you can study cytoskeletal and organelle structure and function in living cells, in real time, and without chemical staining. You can monitor the location of a protein of interest relative to a given subcellular structure by labeling both the protein and the structure with separate fluorescent proteins.

The Subcellular Localization Vectors encode fusions of fluorescent protein variants and localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants. They are ideal for multiplex labeling experiments.

Photoactivatable mCherry Vectors

PAmCherry is a photoactivatable mutant of mCherry that is non-fluorescent until it is exposed to 350–400 nm light. By selecting which cellular regions to activate, you can track organelles against a dark background. PAmCherry subcellular localization vectors are available that target PAmCherry to the cell membrane, mitochondria, actin, or tubulin, respectively. Observe photoactivated PAmCherry with the same filter sets used for other red fluorescent proteins, such as DsRed variants and mCherry.

Lenti-X Actin Dynamics Monitoring Kit

This kit is designed to monitor the highly dynamic behavior of the actin filament system in live cells. The kit includes lentiviral vectors encoding actin fusions to DD-AcGFP1 (green, destabilized) and mCherry (red), and the DD's stabilizing ligand Shield1. DD-AcGFP1-Actin is continuously targeted for degradation in the cell by the proteasomes, unless the cells are cultured in medium containing the stabilizing ligand Shield1. By contrast, mCherry-Actin (which does not contain the DD) has normal stability upon expression, and is constitutively present in the cell.

Adding and removing Shield1 creates a “pulse-chase” like set of conditions which allow you to monitor polymerization of actin monomers as newly synthesized DD-AcGFP1-Actin that is stabilized by Shield1 (green) is integrated into the existing (red) mCherry-Actin actin filament network.

Autophagy Sensor Vector

pAutophagSENSE is a mammalian expression vector that allows you to monitor the process of autophagy. pAutophagSENSE encodes a fusion of the green fluorescent protein AcGFP1 and the mouse LC3 protein. In cells not undergoing autophagy, the AcGFP1-LC3 fusion protein is evenly distributed in the cytoplasm of a transfected cell. However, if a cell is undergoing autophagy via the formation of autophagosomes, the AcGFP1-LC3 fusion protein is incorporated into autophagosomes. This process can be monitored by following the redistribution of the green fluorescent fusion protein from the cytosol to the forming autophagosomes. The readout is a change from an even cytosolic distribution of green fluorescence in cells to a punctate pattern representing the forming autophagosomes. pAutophagSENSE saves you money compared to other autophagy detection systems, because it is vector-based—not a consumable you have to buy over and over again.

At-A-Glance Documents Images & Data Resources

Features

  • Localize fluorescence to specific organelles or structures in living cells
  • Visualize biological processes as they occur
  • Dual- or triple-label cells with different fluorescent proteins
  • Switch on photoactivatable mCherry in a subset of organelles
  • Save money with the only vector-based autophagy detection system—no need to buy antibodies, baculovirus, or dyes repeatedly
  • Many of these subcellular markers are also available as ready-to-use lentivirus particles

Applications

  • Protein localization studies
  • Subcellular analysis (organelles, cell compartments)
  • Studying actin dynamics in the cell
  • Tracking sub-populations of organelles against a dark background using photoactivatable PAmCherry vectors
  • Monitor autophagosome formation in live cells

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
631076 Lenti-X™ Actin Dynamics Monitoring Kit Each $850.00 License Statements *
632453 pAcGFP1-Actin Vector 20 ug $574.00 License Statements *
632490 pAcGFP1-Endo Vector 20 ug $574.00 License Statements *
632510 pAcGFP1-F Hyg Vector 20 ug $574.00 License Statements *
632511 pAcGFP1-F Vector 20 ug $574.00 License Statements *
632464 pAcGFP1-Golgi Vector 20 ug $574.00 License Statements *
632509 pAcGFP1-Mem Hyg Vector 20 ug $574.00 License Statements *
632491 pAcGFP1-Mem Vector 20 ug $574.00 License Statements *
632432 pAcGFP1-Mito Vector 20 ug $574.00 License Statements *
632431 pAcGFP1-Nuc Vector 20 ug $574.00 License Statements *
632488 pAcGFP1-Tubulin Vector 20 ug $574.00 License Statements *
632583 pAutophagSENSE™ Vector Each $574.00 License Statements
632479 pDsRed-Monomer-Actin Vector 20 ug $574.00 License Statements *
632514 pDsRed-Monomer-F Hyg Vector 20 ug $574.00 License Statements *
632493 pDsRed-Monomer-F Vector 20 ug $574.00 License Statements *
632480 pDsRed-Monomer-Golgi Vector 20 ug $574.00 License Statements *
632513 pDsRed-Monomer-Mem Hyg Vector 20 ug $574.00 License Statements *
632512 pDsRed-Monomer-Mem Vector 20 ug $574.00 License Statements *
632419 pDsRed2-Bid Vector 20 ug $574.00 License Statements *
632409 pDsRed2-ER Vector 20 ug $574.00 License Statements *
632421 pDsRed2-Mito Vector 20 ug $574.00 License Statements *
632408 pDsRed2-Nuc Vector 20 ug $574.00 License Statements *
632418 pDsRed2-Peroxi Vector 20 ug $574.00 License Statements *
632434 pHcRed1-Mito Vector 20 ug $574.00 License Statements *
632433 pHcRed1-Nuc Vector 20 ug $574.00 License Statements *
632515 pIRES2-AcGFP1-Nuc Vector 20 ug $574.00 License Statements *
631078 pLVX-mCherry-Actin Vector 10 ug $676.00 License Statements *
632589 pPAmCherry-Actin Vector 10 ug $574.00 License Statements *
632588 pPAmCherry-Mem Vector 10 ug $574.00 License Statements *
632591 pPAmCherry-Mito Vector 10 ug $574.00 License Statements *
632590 pPAmCherry-Tubulin Vector 10 ug $574.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

 

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