632192
DD-ZsGreen1 Reporter System
Each
USD $569.00
The DD-ZsGreen1 Reporter System includes the pDD-ZsGreen1 Reporter vector and Shield1. The pDD-ZsGreen1 Reporter is a promoterless vector designed to insert your promoter of interest upstream of the green fluorescent protein ZsGreen1, tagged at its N-terminus with the ProteoTuner destabilization domain (DD). The DD causes the reporter protein to be rapidly targeted to and degraded by proteasomes. This very efficient and controllable destabilization method will minimize any reporter background fluorescence from leaky promoters prior to promoter activation.
To analyze promoter activity, a candidate inducer is added to the medium along with Shield1, which effectively stabilizes the reporter protein and allows it to accumulate. As a result, only the reporter molecules expressed during promoter induction will contribute to the fluorescence signal, providing a considerably higher signal-to-noise ratio than that obtained with non-destabilized or constitutively destabilized reporter systems.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632191
DD-AmCyan1 Reporter System
Each
USD $569.00
The DD-AmCyan1 Reporter System includes the pDD-AmCyan1 Reporter vector and Shield1. The pDD-AmCyan1 Reporter is a promoterless vector that allows you to insert your promoter of interest upstream of the cyan fluorescent protein AmCyan1, tagged at its N-terminus with the ProteoTuner destabilization domain (DD). The DD causes the reporter protein to be rapidly targeted to and degraded by proteasomes. This very efficient and controllable destabilization method will minimize any reporter background fluorescence from leaky promoters prior to promoter activation.
To analyze promoter activity, a candidate inducer is added to the medium along with Shield1, which effectively stabilizes the reporter protein and allows it to accumulate. As a result, only the reporter molecules expressed during promoter induction will contribute to the fluorescence signal, providing a considerably higher signal-to-noise ratio than that obtained with non-destabilized or constitutively destabilized reporter systems.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632190
DD-tdTomato Reporter System
Each
USD $569.00
The DD-tdTomato Reporter System includes the pDD-tdTomato Reporter vector and Shield1. The pDD-tdTomato Reporter is a promoterless vector that allows you to insert your promoter of interest upstream of the red fluorescent protein tdTomato, tagged at its N-terminus with the ProteoTuner destabilization domain (DD). The DD causes the reporter protein to be rapidly targeted to and degraded by proteasomes. This very efficient and controllable destabilization method will minimize background fluorescence from leaky promoters prior to promoter activation.
To analyze promoter activity, a candidate inducer is added to the medium along with Shield1, which effectively stabilizes the reporter protein and allows it to accumulate. As a result, only the reporter molecules expressed during promoter induction will contribute to the fluorescence signal, providing a considerably higher signal-to-noise ratio than that obtained with non-destabilized or constitutively destabilized reporter systems.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631753
Lenti-X™ DD Red Reporter System
Each
USD $1096.00
The Lenti-X DD Red Reporter System includes the Lenti-X DD-tdTomato Vector Set and Shield1. The Lenti-X DD-tdTomato Vector Set includes two lentiviral expression vectors (a reporter vector and a control vector) that produce high titers of recombinant lentivirus, which can efficiently transduce both dividing and nondividing mammalian cells. The system provides enough reagents for 16 packaging reactions. The pLVX-DD-tdTomato Reporter Vector is a promoterless vector that allows you to insert your promoter of interest upstream of the red fluorescent protein tdTomato, tagged at its N-terminus with the ProteoTuner destabilization domain (DD). In the absence of the membrane-permeant ligand Shield1, the DD causes the reporter protein to be rapidly targeted to and degraded by proteasomes. This very efficient and controllable destabilization method minimizes reporter background fluorescence from leaky promoters prior to promoter activation. To analyze promoter activity, a candidate inducer is added to the medium along with Shield1, which binds to the DD tag and thereby stabilizes the reporter protein and allows it to accumulate. As a result, only the reporter molecules expressed during promoter induction will contribute to the fluorescence signal, providing a considerably higher signal-to-noise ratio than that obtained with nondestabilized or constitutively destabilized reporter systems. Cells used to monitor uninduced promoters (e.g., the negative control) will be treated with Shield1 alone.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631751
Lenti-X™ DD Green Reporter System
Each
USD $1096.00
The Lenti-X DD Green Reporter System includes the Lenti-X DD-ZsGreen1 Vector Set and Shield1. The Lenti-X DD-ZsGreen1 Vector Set includes two lentiviral expression vectors (a reporter vector and a control vector) that produce high titers of recombinant lentivirus, which can efficiently transduce both dividing and nondividing mammalian cells. The system provides enough reagents for 16 packaging reactions. The pLVX-DD-ZsGreen1 Reporter Vector is a promoterless vector that allows you to insert your promoter of interest upstream of the green fluorescent protein ZsGreen1, tagged at its N-terminus with the ProteoTuner destabilization domain (DD). In the absence of the membrane-permeant ligand Shield1, the DD causes the reporter protein to be rapidly targeted to and degraded by proteasomes. This very efficient and controllable destabilization method minimizes reporter background fluorescence from leaky promoters prior to promoter activation. To analyze promoter activity, a candidate inducer is added to the medium along with Shield1, which binds to the DD tag and thereby stabilizes the reporter protein and allows it to accumulate. As a result, only the reporter molecules expressed during promoter induction will contribute to the fluorescence signal, providing a considerably higher signal-to-noise ratio than that obtained with nondestabilized or constitutively destabilized reporter systems. Cells used to monitor uninduced promoters (e.g., the negative control) will be treated with Shield1 alone.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631748
Lenti-X™ DD Cyan Reporter System
Each
USD $1096.00
The Lenti-X DD Cyan Reporter System includes the Lenti-X DD-AmCyan1 Vector Set and Shield1. The Lenti-X DD-AmCyan1 Vector Set includes two lentiviral expression vectors (a reporter vector and a control vector) that produce high titers of recombinant lentivirus, which can efficiently transduce both dividing and nondividing mammalian cells. The system provides enough reagents for 16 packaging reactions. The pLVX-DD-AmCyan1 Reporter Vector is a promoterless vector that allows you to insert your promoter of interest upstream of the cyan fluorescent protein AmCyan1, tagged at its N-terminus with the ProteoTuner destabilization domain (DD). In the absence of the membrane-permeant ligand Shield1, the DD causes the reporter protein to be rapidly targeted to and degraded by proteasomes. This very efficient and controllable destabilization method minimizes reporter background fluorescence from leaky promoters prior to promoter activation.To analyze promoter activity, a candidate inducer is added to the medium along with Shield1, which binds to the DD tag and thereby stabilizes the reporter protein and allows it to accumulate. As a result, only the reporter molecules expressed during promoter induction will contribute to the fluorescence signal, providing a considerably higher signal-to-noise ratio than that obtained with nondestabilized or constitutively destabilized reporter systems. Cells used to monitor uninduced promoters (e.g., the negative control) will be treated with Shield1 alone.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631089
CRE DD Cyan Reporter System
Each
USD $569.00
The CRE DD Cyan Reporter System is designed to monitor cAMP response element binding protein (CREB) activity in mammalian cells, with minimal background signal. It includes the pCRE-DD-AmCyan1 Reporter vector and Shield1.
pCRE-DD-AmCyan1 encodes a cyan fluorescent protein reporter tagged at its N-terminus with the ProteoTuner destabilization domain (DD), and under the control of the CRE promoter. The DD causes the DD-AmCyan1 reporter to be rapidly targeted to and degraded by proteasomes. This minimizes background fluorescence from leaky promoters prior to promoter activation.
To monitor CREB activity, a candidate inducer is added to the medium simultaneously with the DD's stabilizing ligand, Shield1. This allows DD-AmCyan1 to accumulate in response to CREB activation. As a result, only the reporter molecules expressed during CRE induction contribute to the fluorescence signal. This system provides a considerably higher signal-to-noise ratio than can be obtained with non-destabilized or constitutively destabilized reporter systems.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631087
CRE DD Red Reporter System
Each
USD $569.00
The CRE DD Red Reporter System is designed to monitor cAMP response element binding protein (CREB) activity in mammalian cells, with minimal background signal. It includes the pCRE-DD-tdTomato Reporter vector and Shield1.
pCRE-DD-tdTomato encodes a red fluorescent protein reporter tagged at its N-terminus with the ProteoTuner destabilization domain (DD), and under the control of the CRE promoter. The DD causes the DD-tdTomato reporter to be rapidly targeted to and degraded by proteasomes. This minimizes background fluorescence from leaky promoters prior to promoter activation.
To monitor CREB activity, a candidate inducer is added to the medium simultaneously with the DD's stabilizing ligand, Shield1. This allows DD-tdTomato to accumulate in response to CREB activation. As a result, only the reporter molecules expressed during CRE induction contribute to the fluorescence signal. This system provides a considerably higher signal-to-noise ratio than can be obtained with non-destabilized or constitutively destabilized reporter systems.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631085
CRE DD Green Reporter System
Each
USD $569.00
The CRE DD Green Reporter System is designed to monitor cAMP response element binding protein (CREB) activity in mammalian cells, with minimal background signal. It includes the pCRE-DD-ZsGreen1 Reporter vector and Shield1.
pCRE-DD-ZsGreen1 encodes a green fluorescent protein reporter tagged at its N-terminus with the ProteoTuner destabilization domain (DD), and under the control of the CRE promoter. The DD causes the DD-ZsGreen1 reporter to be rapidly targeted to and degraded by proteasomes. This minimizes background fluorescence from leaky promoters prior to promoter activation.
To monitor CREB activity, a candidate inducer is added to the medium simultaneously with the DD's stabilizing ligand, Shield1. This allows DD-ZsGreen1 to accumulate in response to CREB activation. As a result, only the reporter molecules expressed during CRE induction contribute to the fluorescence signal. This system provides a considerably higher signal-to-noise ratio than can be obtained with non-destabilized or constitutively destabilized reporter systems.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631083
NFkappaB DD Cyan Reporter System
Each
USD $569.00
The NFk B DD Cyan Reporter System is designed to monitor NFk B activation in mammalian cells, with minimal background signal. It includes the pNFkB-DD-AmCyan1 Reporter vector and Shield1.
pNFk B-DD-AmCyan1 encodes a cyan fluorescent protein reporter tagged at its N-terminus with the ProteoTuner destabilization domain (DD), and under the control of the NFk B promoter. The DD causes the DD-AmCyan1 reporter to be rapidly targeted to and degraded by proteasomes. This minimizes background signal from leaky promoters prior to promoter activation.
To monitor NFk B activity, a candidate inducer is added to the medium simultaneously with the DD's stabilizing ligand, Shield1. This allows DD-AmCyan1 to accumulate in response to NFk B activation. As a result, only the reporter molecules expressed during NFk B induction contribute to the fluorescence signal. This system provides a considerably higher signal-to-noise ratio than can be obtained with non-destabilized or constitutively destabilized reporter systems.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631081
NFkappaB DD Red Reporter System
Each
USD $569.00
The NFk B DD Red Reporter System is designed to monitor NFk B activation in mammalian systems, with minimal background signal. It includes the pNFk B-DD-tdTomato Reporter vector and Shield1.
pNFk B-DD-tdTomato encodes a red fluorescent protein reporter tagged at its N-terminus with the ProteoTuner destabilization domain (DD), and under the control of the NFk B promoter. The DD causes the DD-tdTomato reporter to be rapidly targeted to and degraded by proteasomes. This minimizes background fluorescence from leaky promoters prior to promoter activation.
To monitor NFk B activity, a candidate inducer is added to the medium simultaneously with the DD's stabilizing ligand, Shield1. This allows DD-tdTomato to accumulate in response to NFk B activation. As a result, only the reporter molecules expressed during NFk B induction contribute to the fluorescence signal. This system provides a considerably higher signal-to-noise ratio than can be obtained with non-destabilized or constitutively destabilized reporter systems.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631079
NFkappaB DD Green Reporter System
Each
USD $569.00
The NFk B DD Green Reporter System is designed to monitor NFkB activation in mammalian cells, with minimal background signal. It includes the pNFk B-DD-ZsGreen1 Reporter vector and Shield1.
pNFk B-DD-ZsGreen1 encodes a green fluorescent protein reporter tagged at its N-terminus with the ProteoTuner destabilization domain (DD), and under the control of the NFk B promoter. The DD causes the DD-ZsGreen1 reporter to be rapidly targeted to and degraded by proteasomes. This minimizes background signal from leaky promoters prior to promoter activation.
To monitor NFk B activity, a candidate inducer is added to the medium simultaneously with the DD's stabilizing ligand, Shield1. This allows DD-ZsGreen1 to accumulate in response to NFk B activation. As a result, only reporter molecules expressed during NFk B induction will contribute to the fluorescence signal. This system provides a considerably higher signal-to-noise ratio than can be obtained with non-destabilized or constitutively destabilized reporter systems.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.