mBanana Fluorescent Protein & ZsYellow1 Fluorescent Protein
Clontech offers two Living Colors yellow fluorescent proteins, ZsYellow1 and mBanana.
mBanana Fluorescent Protein
mBanana is a Fruit Fluorescent Protein which was developed in Dr. Roger Tsien’s lab (1-3) by directed mutagenesis of mRFP1, a monomeric mutant of DsRed (4). Fruit Fluorescent Proteins such as mBanana have demonstrated stable expression and perform successfully in numerous fusion applications.
ZsYellow1 Fluorescent Protein
ZsYellow1 has a true yellow emission, making it ideal for multicolor applications. Like all of Clontech's Living Colors Fluorescent Proteins, ZsYellow1 can be detected in cells without adding cofactors or substrates, which makes it ideal for use in live cell assays. The gene for ZsYellow1 has been human codon-optimized to enhance its translation in mammalian cells, and adapted for higher solubility, brighter emission, and rapid chromophore maturation (8–12 hours).
The ZsYellow1 fluorescent protein can be used as a molecular tag or as a reporter to visualize, track, and quantify cellular processes including protein synthesis and turnover, protein translocation, gene induction, and cell lineage. Because its excitation and emission spectra are distinct from our other fluorescent proteins, ZsYellow1 can be used for multiplex applications—that is, to simultaneously detect two or more events in the same cell or cell population. For example, it can be used in two- and three-color analyses with AmCyan1 and/or HcRed1. Like all of our Living Colors Fluorescent Proteins, ZsYellow1 is ideal for monitoring gene expression and protein localization in vivo, in situ, and in realtime.