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Products >  Fluorescent_Proteins_and_Reporters >  Fluorescent_Proteins >  Fusions >  Yellow

mBanana Fluorescent Protein & ZsYellow1 Fluorescent Protein

Clontech offers two Living Colors yellow fluorescent proteins, ZsYellow1 and mBanana.

mBanana Fluorescent Protein

mBanana is a Fruit Fluorescent Protein which was developed in Dr. Roger Tsien’s lab (1-3) by directed mutagenesis of mRFP1, a monomeric mutant of DsRed (4). Fruit Fluorescent Proteins such as mBanana have demonstrated stable expression and perform successfully in numerous fusion applications.

ZsYellow1 Fluorescent Protein

ZsYellow1 has a true yellow emission, making it ideal for multicolor applications. Like all of Clontech's Living Colors Fluorescent Proteins, ZsYellow1 can be detected in cells without adding cofactors or substrates, which makes it ideal for use in live cell assays. The gene for ZsYellow1 has been human codon-optimized to enhance its translation in mammalian cells, and adapted for higher solubility, brighter emission, and rapid chromophore maturation (8–12 hours).

The ZsYellow1 fluorescent protein can be used as a molecular tag or as a reporter to visualize, track, and quantify cellular processes including protein synthesis and turnover, protein translocation, gene induction, and cell lineage. Because its excitation and emission spectra are distinct from our other fluorescent proteins, ZsYellow1 can be used for multiplex applications—that is, to simultaneously detect two or more events in the same cell or cell population. For example, it can be used in two- and three-color analyses with AmCyan1 and/or HcRed1. Like all of our Living Colors Fluorescent Proteins, ZsYellow1 is ideal for monitoring gene expression and protein localization in vivo, in situ, and in realtime.

Anti-mBanana and -ZsYellow1 Antibodies

We offer monoclonal and polyclonal antibodies to detect mBanana, and a polyclonal anti-ZsYellow1 Antibody.

At-A-Glance Documents Images & Data Resources

Features

  • Bright yellow fluorescent proteins
  • mBanana excitation and emission maxima are 540 and 553 nm, respectively
  • ZsYellow1 excitation and emission maxima are 529 and 539 nm, respectively

Applications

  • Fusions
  • Protein localization
  • General reporter for mammalian cells
  • Monitoring transfection efficiencies

References

  1. Shaner, N. C. et al. (2004) Nature Biotechnol. 22(12):1567–1572.
  2. Wang, L. et al. (2004) Proc. Nat. Acad. Sci. USA. 101(48):16745–16749.
  3. Shu, X. et al. (2006) Biochemistry 45(32):9639–9647.
  4. Campbell, R. E. et al. (2002) Proc. Nat. Acad. Sci. USA. 99(12):7877–7882.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
Products
Cat. # Product Package Size Price License # of Units Select
630050 Reef Coral Fluorescent Protein Vector Set Each $5,311.00 License Statements *
632444 pZsYellow1-C1 Vector 20 ug $574.00 License Statements *
632445 pZsYellow1-N1 Vector 20 ug $574.00 License Statements *
632528 pmBanana Vector 20 ug $516.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

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