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Fluorescent Proteins & Reporters


Products >  Fluorescent_Proteins_and_Reporters >  Fluorescent_Proteins >  Fusions >  Red

Red Fluorescent Proteins—mCherry, tdTomato, DsRed, AsRed & mStrawberry for Fusions

Living Colors mCherry has been successfully fused to many proteins and widely used for quantitative imaging techniques including fluorescence resonance energy transfer (FRET). mCherry is one of the Fruit Fluorescent Proteins, which were developed in Dr. Roger Tsien’s lab (1–3) by directed mutagenesis of mRFP1, a monomeric mutant of DsRed (4).

tdTomato

Living Colors tdTomato (also developed in Dr. Roger Tsien’s lab) is a genetic fusion of two copies of the dTomato gene (4) which was specifically designed for low aggregation. Its tandem dimer structure plays an important role in the exceptional brightness of tdTomato. Because tdTomato forms an intramolecular dimer, it behaves like a monomer, and has been used successfully for N- and C- terminal fusions. tdTomato’s emission wavelength (581 nm) and brightness make it ideal for live animal imaging studies. tdTomato has been detected as deep as 1 cm below the surface (5).

DsRed-Express2 & DsRed-Express

Living Colors DsRed-Express is a rapidly maturing variant of Discosoma sp. red fluorescent protein with mutations that enhance its solubility, reduce its green emission, and accelerate its maturation (6). DsRed-Express2 has even higher solubility, and was designed to be better suited for cell and stem cell applications (7).

DsRed-Monomer

Living Colors DsRed-Monomer is a true monomeric mutant of our red fluorescent protein from Discosoma sp. reef coral, which makes it the optimal choice for use as a red fluorescent fusion tag. DsRed-Monomer has been expressed as a fusion with a large panel of diverse proteins with diverse functions and subcellular locations. The localization of the resulting tagged protein was monitored, and all the tested proteins localized properly.

Dual-Color, Stable Cell Lines

With the pDsRed-Monomer-Hyg-N1/C1 and pAcGFP1-Hyg-N1/C1 vectors, you can establish stable cell lines coexpressing AcGFP1 and DsRed-Monomer fusion proteins, by selecting with both hygromycin and neomycin. Target genes of interest can be easily transferred from our standard pDsRed-Monomer-N1/C1 and pAcGFP1-N1/C1 vectors containing the neomycin selection cassette into the corresponding hygromycin-resistant (Hygr) N1 or C1 vectors, because the two vector sets contain identical multiple cloning sites.

DsRed2 & AsRed2

Living Colors DsRed2 and AsRed2 retain the benefits typical of red fluorescent proteins, such as a high signal-to-noise ratio and distinct spectral properties for use in multicolor labeling experiments.

mStrawberry

Living Colors mStrawberry is a Fruit Fluorescent Protein which was developed in Dr. Roger Tsien’s lab (1-3) by directed mutagenesis of mRFP1, a monomeric mutant of DsRed (4). It's well-tolerated as a fusion protein in a wide variety of applications.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Bright red fluorescent proteins suited for fusion applications
  • N & C terminal tagging vectors
  • Plasmid or viral delivery vectors
Fluorescent Protein Excitation Maximum (nm)Emission Maximum (nm)
mCherry587610
tdTomato554581
DsRed-Monomer557592
DsRed-Express2554591
DsRed-Express554586
DsRed2563582
AsRed2576592
mStrawberry574596

Applications

  • Protein localization studies
  • General reporter for mammalian cells
  • Monitoring transfection efficiencies

References

  1. Shaner, N. C. et al. (2004) Nature Biotechnol. 22(12):1567–1572.
  2. Wang, L. et al. (2004) Proc. Nat. Acad. Sci. USA. 101(48):16745–16749.
  3. Shu, X. et al. (2006) Biochemistry 45(32):9639–9647.
  4. Campbell, R. E. et al. (2002) Proc. Nat. Acad. Sci. USA. 99(12):7877–7882.
  5. Winnard Jr., P. T. et al. (2006) Neoplasia 8(10):796–806.
  6. Bevis, B. J. and Glick, B. S. (2002) Nat. Biotechnol. 20(1):83–87.
  7. Strack, R. L. et al. (2008) Nat Methods. 5(11):955–957.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 

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