Dendra2 Photoswitchable Fluorescent Protein for Fusions
Living Colors Dendra2 is a monomeric, green-to-red photoswitchable fluorescent protein derived from octocoral Dendronephthya sp. Dendra2 is an ideal tool for real-time tracking protein dynamics (movement, degradation, etc.) and monitoring selective cell fate.
After complete photoconversion, the red fluorescence of Dendra2 increases 150–300 times, whereas the level of green fluorescence becomes 10–15 times lower. Thus, the increase in the red-to-green fluorescence ratio results in ~4000-fold contrast. This provides a molecular tool to simultaneously track both the movement of the activated protein and its replacement with the non-activated form.
Excellent Performance in Fusion Applications
Dendra2 has been successfully fused to a variety of proteins including cytoplasmic beta-actin, BH3 interacting domain death agonist (BID), nucleolar protein fibrillarin, vimentin, and alpha-tubulin.