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Fluorescent Proteins & Reporters


Products >  Fluorescent_Proteins_and_Reporters >  Fluorescent_Proteins >  Fusions >  Orange

mOrange Fluorescent Protein

Living Colors mOrange and mOrange2 are extremely bright orange fluorescent protein monomers which can be used as tags or reporters. Both mOrange fluorescent proteins are mutants derived from mRFP1, a monomeric mutant of DsRed, which was developed in Dr. Roger Tsien’s lab (1–3) by directed mutagenesis (4). 

Fusions with mOrange2 Fluorescent Protein

Generate N- or C-terminal fusion proteins to express and visualize a protein of interest fused to mOrange2 in mammalian cells. 

Anti-mOrange Antibody

We offer a monoclonal antibody and a polyclonal antibody that recognize mOrange2 and mOrange.

At-A-Glance Documents Images & Data Resources

Features

  • Bright orange fluorescent protein monomers
  • mOrange2 excitation and emission maxima are 549 and 565 nm, respectively
  • mOrange excitation and emission maxima are 548 and 562 nm, respectively

Applications

  • Fusions
  • Tracking cell, organelle, and protein movement
  • Protein localization studies
  • General reporter for mammalian cells
  • Monitoring transfection efficiencies

References

  1. Shaner, N. C. et al. (2004) Nature Biotechnol. 22(12):1567–1572. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.
  2. Wang, L. et al. (2004) Proc. Nat. Acad. Sci. USA. 101(48):16745–16749. Evolution of new nonantibody proteins via iterative somatic hypermutation.
  3. Shu, X. et al. (2006) Biochemistry 45(32):9639–9647. Novel chromophores and buried charges control color in mFruits.
  4. Campbell, R. E. et al. (2002) Proc. Nat. Acad. Sci USA. 99(12):7877–7882. A monomeric red fluorescent protein.
  5. Shaner, N. C. et al. (2008) Nature Methods. 5(6): 545–551. Improving the photostability of bright monomeric orange and red fluorescent proteins.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
Products
Cat. # Product Package Size Price License # of Units Select
632550 pmOrange2-C1 Vector 20 ug $574.00 License Statements *
632549 pmOrange2-N1 Vector 20 ug $574.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

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