Generate N- or C-terminal GFP fusion proteins to express and visualize a protein of interest fused to a green fluorescent protein in mammalian cells.
ZsGreen1 Fusions
Living Colors ZsGreen1 is a bright green fluorescent protein that was derived from a reef coral belonging to class Anthozoa, which has been modified for higher solubility, brighter emission, and rapid chromophore maturation (8–12 hours) compared to the unmodified protein.
AcGFP1 Fusions
Living Colors AcGFP1 is an engineered, fluorescent mutant of the wild-type protein derived from the jellyfish Aequorea coerulescens. It is a true monomer that does not form dimers as has been reported for AvGFP and EGFP (1, 2). Its open reading frame has been human codon optimized to increase translational efficiency and expression levels. AcGFP1 has been widely validated as a fusion tag and in cell-based assays that monitor protein subcellular trafficking.
AcGFP1 Vector Sets
Each vector in the set contains the MCS in a different reading frame with respect to AcGFP1. Both N- and C-terminal AcGFP1 vector sets are available.
Dual-Color, Stable Cell Lines
With the pDsRed-Monomer-Hyg-N1/C1 and pAcGFP1-Hyg-N1/C1 vectors, you can establish stable cell lines coexpressing AcGFP1 and DsRed-Monomer fusion proteins, by selecting with both hygromycin and neomycin. Target genes of interest can be easily transferred from our standard pDsRed-Monomer-N1/C1 and pAcGFP1-N1/C1 vectors containing the neomycin selection cassette into the corresponding hygromycin-resistant (Hygr) N1 or C1 vectors, because the two vector sets contain identical multiple cloning sites.
