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Fluorescent Proteins & Reporters


Products >  Fluorescent_Proteins_and_Reporters >  Fluorescent_Proteins >  Fusions >  Green

Green Fluorescent Protein (GFP) Fusions- AcGFP1 & ZsGreen1

Generate N- or C-terminal GFP fusion proteins to express and visualize a protein of interest fused to a green fluorescent protein in mammalian cells.

ZsGreen1 Fusions

Living Colors ZsGreen1 is a bright green fluorescent protein that was derived from a reef coral belonging to class Anthozoa, which has been modified for higher solubility, brighter emission, and rapid chromophore maturation (8–12 hours) compared to the unmodified protein.

AcGFP1 Fusions

Living Colors AcGFP1 is an engineered, fluorescent mutant of the wild-type protein derived from the jellyfish Aequorea coerulescens. It is a true monomer that does not form dimers as has been reported for AvGFP and EGFP (1, 2). Its open reading frame has been human codon optimized to increase translational efficiency and expression levels. AcGFP1 has been widely validated as a fusion tag and in cell-based assays that monitor protein subcellular trafficking.

AcGFP1 Vector Sets

Each vector in the set contains the MCS in a different reading frame with respect to AcGFP1. Both N- and C-terminal AcGFP1 vector sets are available.

Dual-Color, Stable Cell Lines

With the pDsRed-Monomer-Hyg-N1/C1 and pAcGFP1-Hyg-N1/C1 vectors, you can establish stable cell lines coexpressing AcGFP1 and DsRed-Monomer fusion proteins, by selecting with both hygromycin and neomycin. Target genes of interest can be easily transferred from our standard pDsRed-Monomer-N1/C1 and pAcGFP1-N1/C1 vectors containing the neomycin selection cassette into the corresponding hygromycin-resistant (Hygr) N1 or C1 vectors, because the two vector sets contain identical multiple cloning sites.

At-A-Glance Documents Images & Data Resources

Features

  • Bright GFP expression vectors, suited for fusion applications
  • ZsGreen1 excitation and emission maxima are 493 and 505 nm, respectively
  • AcGFP1 excitation and emission maxima are 475 and 505 nm, respectively
  • N & C terminal tagging vectors
  • Plasmid or viral delivery vectors

Applications

  • Protein localization studies
  • General reporter for mammalian cells
  • Monitoring transfection efficiencies

References

  1. Jain, R. K., et al. (2001) Biochem. J. 360(Pt. 3):645–649.
  2. Chen, Y., et al. (2002) Biophys. J. 32(1):133–144.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
Products
Cat. # Product Package Size Price License # of Units Select
632426 AcGFP1 Vector Set Each $1,102.00 License Statements *
630050 Reef Coral Fluorescent Protein Vector Set Each $5,311.00 License Statements *
632500 pAcGFP1-C In-Fusion® Ready Vector 1 ug $396.00 License Statements *
632470 pAcGFP1-C1 Vector 20 ug $574.00 License Statements *
632481 pAcGFP1-C2 Vector 20 ug $574.00 License Statements *
632482 pAcGFP1-C3 Vector 20 ug $574.00 License Statements *
632492 pAcGFP1-Hyg-C1 Vector 20 ug $574.00 License Statements *
632489 pAcGFP1-Hyg-N1 Vector 20 ug $574.00 License Statements *
632501 pAcGFP1-N In-Fusion® Ready Vector 1 ug $396.00 License Statements *
632485 pAcGFP1-N Vector Set Each $1,002.00 License Statements *
632469 pAcGFP1-N1 Vector 20 ug $574.00 License Statements *
632483 pAcGFP1-N2 Vector 20 ug $574.00 License Statements *
632484 pAcGFP1-N3 Vector 20 ug $574.00 License Statements *
631974 pEF1alpha-AcGFP1-C1 Vector 10 ug $541.00 License Statements *
631973 pEF1alpha-AcGFP1-N1 Vector 10 ug $541.00 License Statements *
632155 pLVX-AcGFP1-C1 Vector 10 ug $676.00 License Statements *
632154 pLVX-AcGFP1-N1 Vector 10 ug $676.00 License Statements *
632566 pLVX-ZsGreen1-C1 Vector 10 ug $676.00 License Statements *
632565 pLVX-ZsGreen1-N1 Vector 10 ug $676.00 License Statements *
632506 pRetroQ-AcGFP1-C1 Vector 20 ug $568.00 License Statements *
632505 pRetroQ-AcGFP1-N1 Vector 20 ug $568.00 License Statements *
632447 pZsGreen1-C1 Vector 20 ug $574.00 License Statements *
632448 pZsGreen1-N1 Vector 20 ug $574.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

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