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Fluorescent Proteins & Reporters


Products >  Fluorescent_Proteins_and_Reporters >  Fluorescent_Proteins >  Fusions >  Far_Red

mPlum Fluorescent Protein, mRaspberry Fluorescent Protein, HcRed1 Fluorescent Protein & E2-Crimson Fluorescent Protein

Far red proteins avoid the natural green autofluorescence found in plants and animals, and are preferred for in vivo imaging.

mPlum Fluorescent Protein & mRaspberry Fluorescent Protein

Living Colors mPlum and mRaspberry are Fruit Fluorescent Proteins which were developed in Dr. Roger Tsien’s lab (1–3) by directed mutagenesis of mRFP1, a monomeric mutant of DsRed (4). They have demonstrated stable expression and perform successfully in numerous fusion applications.

mPlum Antibody, mRaspberry Antibody

We offer anti-mPlum and mRaspberry antibodies (monoclonal and polyclonal).

HcRed1 Fluorescent Protein

Living Colors HcRed is a far-red fluorescent protein derived from a nonfluorescent chromoprotein found in the sea anemone Heteractis crispa. The coding sequence of the HcRed1 fluorescent protein is human codon-optimized for enhanced expression in mammalian cells. HcRed1 has a low tendency to form aggregates in living cells, and can be detected just 16 hours after transfection by flow cytometry, a time frame which is comparable with EGFP. We offer an anti-HcRed1 antibody.

E2-Crimson Fluorescent Protein

Living Colors E2-Crimson is a bright far-red fluorescent protein that was designed for in vivo applications involving sensitive cells such as primary cells and stem cells. E2-Crimson was derived from DsRed-Express2, and retains its rapid maturation (half time of 26 minutes at 37°C), high photostability, high solubility, and low cytotoxicity (5). We offer monoclonal and polyclonal anti-E2-Crimson antibodies.

At-A-Glance Documents Images & Data Resources

Features

  • Far red fluorescent proteins
  • mPlum excitation and emission maxima: 590 and 649 nm, respectively
  • mRaspberry fluorescent protein excitation and emission maxima: 598 and 625 nm, respectively
  • HcRed1 fluorescent protein excitation and emission maxima: 588 and 618 nm, respectively
  • E2-Crimson fluorescent protein excitation and emission maxima: 611 and 646 nm, respectively

Applications

References

  1. Shaner, N. C. et al. (2004) Nature Biotechnol. 22(12):1567–1572.
  2. Wang, L. et al. (2004) Proc. Nat. Acad. Sci. USA. 101(48):16745–16749.
  3. Shu, X. et al. (2006) Biochemistry 45(32):9639–9647.
  4. Campbell, R. E. et al. (2002) Proc. Nat. Acad. Sci. USA. 99(12):7877–7882.
  5. Strack, R. L. et al. (2009) Biochemistry 48(35):8279–8281.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
Products
Cat. # Product Package Size Price License # of Units Select
632555 pE2-Crimson-C1 Vector 20 ug $574.00 License Statements *
632554 pE2-Crimson-N1 Vector 20 ug $574.00 License Statements *
632415 pHcRed1-C1 Vector 20 ug $574.00 License Statements *
632424 pHcRed1-N1/1 Vector 20 ug $574.00 License Statements *
632527 pmPlum Vector 20 ug $516.00 License Statements *
632526 pmRaspberry Vector 20 ug $516.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

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