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Fluorescent Proteins & Reporters


Products >  Fluorescent_Proteins_and_Reporters >  Coexpression >  Bidirectional_Promoter

Bidirectional Promoter Vectors

The pBI bidirectional vectors are designed to constitutively express a protein of interest and a reporter protein (or two proteins of interest). Expression is driven by constitutively active bidirectional human cytomegalovirus promoters (PminCMV). The gene(s) of interest must contain an initiation codon and a stop codon.

At-A-Glance Documents Images & Data Resources

Features

  • Constitutively express a protein of interest and a reporter protein (or two proteins of interest)
  • Choose from green or red fluorescent reporters
  • pBI-CMV1 allows the constitutive expression of two proteins of interest.
  • pBI-CMV2 allows you to express a protein of interest and AcGFP1, a monomeric green fluorescent protein derived from Aequorea coerulescens.
  • pBI-CMV3 allows you to express a protein of interest and ZsGreen1, an extremely bright green fluorescent protein derived from Zoanthus sp. reef coral.
  • pBI-CMV4 allows you to express a protein of interest and DsRed2, a red fluorescent protein derived from Discosoma sp. reef coral.
  • pBI-CMV5 allows you to express a protein of interest and firefly luciferase. pBI-CMV5 is not sold separately. It is sold as part of the Bidirectional Secreted Luciferase System, together with its reporter assay.
  • To generate stable clones using these bidirectional vectors, we recommend cotransfection with a linearized selection marker or a vector containing an antibiotic selection marker.

Applications

  • Constitutive expression of a protein of interest and a reporter protein

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
631757 Bidirectional Secreted Luciferase System Each $550.00 License Statements
631630 pBI-CMV1 Vector 20 ug $541.00  
631631 pBI-CMV2 Vector 20 ug $541.00 License Statements *
631632 pBI-CMV3 Vector 20 ug $541.00 License Statements *
631633 pBI-CMV4 Vector 20 ug $541.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

Required Products
Cat. # Product Package Size Price # of Units Select
631625 Linear Hygromycin Marker 2 ug $168.00
631626 Linear Puromycin Marker 2 ug $168.00
 

 

 


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