The pBI bidirectional vectors are designed to constitutively express a protein of interest and a reporter protein (or two proteins of interest) at similar levels (unlike IRES vectors, in which the expression of the proteins that are upstream and downstream of the IRES sequence varies). In the pBI bidirectional vectors, expression is driven by constitutively active human cytomegalovirus promoters (PminCMV). The gene(s) of interest must contain an initiation codon and a stop codon.
- pBI-CMV1 allows the constitutive expression of two proteins of interest.
- pBI-CMV2 allows you to express a protein of interest and AcGFP1, a monomeric green fluorescent protein derived from Aequorea coerulescens.
- pBI-CMV3 allows you to express a protein of interest and ZsGreen1, an extremely bright green fluorescent protein derived from Zoanthus sp. reef coral.
- pBI-CMV4 allows you to express a protein of interest and DsRed2, a red fluorescent protein derived from Discosoma sp. reef coral.
- pBI-CMV5 allows you to express a protein of interest and firefly luciferase. pBI-CMV5 is not sold separately. It is sold as part of the Bidirectional Secreted Luciferase System, together with its reporter assay.
To generate stable clones using these bidirectional vectors, we recommend cotransfection with a linearized selection marker or a vector containing an antibiotic selection marker.