The Great EscAPe SEAP 2.0 System uses a secreted alkaline phosphatase assay to analyze cis-acting DNA sequences and trans-acting factors. The kinetics of gene expression can easily be studied with this secreted alkaline phosphatase assay by repeatedly sampling the same culture. The kit has an extremely low background signal, with a wide dynamic range and high sensitivity. Furthermore, the robust signal is extremely stable over time. Cells can be studied further after the secreted alkaline phosphatase assay using Northern blots, RNase protection assays, Western blots and other methods.
The Great EscAPe SEAP Fluorescence Detection Kit uses the fluorescent substrate 4-methylumbelliferyl phosphate (MUP) and has sensitivity comparable to firefly luciferase assays. Like the chemiluminescence assay, the secreted alkaline phosphatase fluorescence assay is linear over a 104-fold range of enzyme concentrations.
Dual Secreted Reporter Assay—SEAP & Luciferase
The Ready-To-Glow Dual Secreted Reporter System has two live cell reporters that do not require cell lysis. In addition to secreted alkaline phosphatase, it includes Metridia luciferase, which has a 2–4 fold higher signal compared to Renilla and firefly luciferases.