ProLabel Kits provide sensitive, chemiluminescence-based assays for detecting and measuring the expression of any recombinant protein of interest. The ProLabel assay is based on enzyme fragment complementation. A 6KDa ProLabel tag encodes an inactive enzyme fragment, which is expressed as an N- or C-terminal tag fused to your protein of interest. When the ProLabel fusion protein is combined with Enzyme Acceptor (EA), supplied in the Detection Kit, the ProLabel tag and the Enzyme Acceptor combine to form a complete, active enzyme that cleaves the chemiluminescent substrate. The resulting signal can be detected and quantified with any standard luminometer. The assay provides a low threshold of detection as well as an excellent dynamic range, allowing you to easily detect changes in protein expression levels.
Our ProLabel Quantitative Expression Vector Set allows you to express your protein of interest as both N- and C- terminal ProLabel fusion proteins. The Vector Set works in conjunction with the ProLabel Chemiluminescent Detection Kit, which provides all of the reagents you need to quickly, easily, and directly measure the expression of your ProLabel fusion protein, even in crude cell lysates. These kits were designed to assay for protein knockdown in RNA interference (RNAi) studies, but they can also be used to create assays for any ProLabel fusion protein.