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Products >  Fluorescent_Proteins_and_Reporters >  Chemiluminescent_Reporters >  Luciferase_Assay >  Secreted_Luciferase

Luciferase Reporter—Ready-To-Glow Secreted Luciferase Assay

The Ready-To-Glow Secreted Luciferase Assay is based on the secreted Metridia luciferase reporter. It combines the advantages of a live-cell assay with the sensitivity of an enzyme-based system.

Our Ready-To-Glow MetLuc Monoclonal Antibody makes it easy to detect Metridia luciferase expressed by mammalian cells, as well as recombinant Metridia luciferase, in amounts down to 20 ng using Western blot analysis.

Benefits of using a live cell assay


Ready-To-Glow Secreted Luciferase Reporter Systems Firefly and Renilla-Based Reporter Systems
One-step protocol, without cell lysis Yes. Reporter molecule is naturally secreted into the media and can be assayed without lysing cells.No.
2–4 fold higher signal than Renilla or firefly assaysYes.No.
Multiple data points per wellYes. Single well can be used for multiple time points, as there is no cell lysis.No.
Multiple compounds/trigger testing on same cells (single transfection)Yes.No.
Removal of background signalYes. Background signal can be reset to zero by removing and replacing the mediaNo.

Available Formats

  • The Ready-To-Glow Secreted Luciferase Reporter System includes a promoterless plasmid and is designed to insert your promoter of interest upstream of the sequence-optimized Metridia secreted luciferase reporter gene.
  • The Lenti-X Ready-To-Glow Secreted Luciferase Reporter System includes a promoterless vector with lentiviral delivery, and is designed to insert your promoter of interest upstream of the sequence-optimized Metridia secreted luciferase reporter gene.
  • The NFkB Secreted Luciferase Reporter System is designed to monitor NFkappaB signal transduction pathways. Adding TNF-alpha, IL-1, or lymphokine receptor stimulants to the cell culture medium induces the binding of transcription factors to the enhancer element, thereby initiating transcription of the secreted Metridia luciferase reporter gene.
  • The CRE Secreted Luciferase Reporter System is designed to monitor the activation of cAMP binding protein (CREB) and cAMP-mediated signal transduction pathways. For example, treatment of cells with cAMP or forskolin activates CREB or ATF to bind CRE, thus initiating transcription of the secreted Metridia luciferase reporter gene.

Dual Secreted Reporter Assay—Luciferase & SEAP

The Ready-To-Glow Dual Secreted Reporter System has two live cell reporters that do not require cell lysis. In addition to the secreted luciferase assay, it includes a secreted version of alkaline phosphatase.

At-A-Glance Documents Images & Data Resources

Features

  • Secreted luciferase assay protocol eliminates cell lysis
  • 2–4 fold higher signal than firefly or Renilla luciferase
  • Single transfection allows multiple time points from same cell
  • Dual, live cell assay includes SEAP as well as secreted luciferase:
    monitor two promoters, or use one reporter as a normalization control

Applications

  • Homogeneous assay protocols
  • Time course studies
  • Test multiple compounds
  • Suitable for downstream experiments with the same cells
  • Use the dual system (which includes secreted alkaline phosphatase, as well as a secreted luciferase assay) to monitor two promoters
  • Use the dual system to monitor one promoter with a built-in normalization control

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
631757 Bidirectional Secreted Luciferase System Each $550.00 License Statements
631746 Lenti-X™ Ready-To-Glow™ Secreted Luciferase Reporter System Each $1,096.00 License Statements *
631739 Ready-To-Glow™ Automation Kit 1,000 Rxns $740.00  
631740 Ready-To-Glow™ Automation Kit 5,000 Rxns $1,560.00  
631745 Ready-To-Glow™ CRE Secreted Luciferase Reporter System Each $330.00 License Statements
631734 Ready-To-Glow™ Dual Secreted Reporter Assay 500 Rxns $648.00  
631735 Ready-To-Glow™ Dual Secreted Reporter Vector Kit 4 x 20 ug $514.00 License Statements
631781 Ready-To-Glow™ MetLuc Monoclonal Antibody 50 ug $410.00  
631743 Ready-To-Glow™ NFkappaB Secreted Luciferase Reporter System Each $330.00 License Statements
631727 Ready-To-Glow™ Secreted Luciferase Reporter Assay 500 Rxns $359.00  
631726 Ready-To-Glow™ Secreted Luciferase Reporter Assay 100 Rxns $102.00  
631728 Ready-To-Glow™ Secreted Luciferase Reporter Assay 1,000 Rxns $507.00  
631730 Ready-To-Glow™ Secreted Luciferase Reporter System 100 Rxns $330.00 License Statements
631731 Ready-To-Glow™ Secreted Luciferase Reporter System 500 Rxns $549.00 License Statements
631732 Ready-To-Glow™ Secreted Luciferase Reporter System 1,000 Rxns $676.00 License Statements
631729 Ready-To-Glow™ Secreted Luciferase pMetLuc Vector Kit 20 ug $287.00 License Statements
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
631781 Ready-To-Glow™ MetLuc Monoclonal Antibody 50 ug $410.00
 
 

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