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Products >  Cloning_and_Competent_Cells >  Mutagenesis_and_Cloning >  Mutagenesis_Cloning_Kits

Site-Directed Mutagenesis: PCR Based Mutagenesis with the In-Fusion HD Cloning System

In-Fusion Cloning Kits enable PCR mediated site-directed DNA mutagenesis. You can add, delete, or replace DNA segments; insert point mutations; insert restriction sites and more using a streamlined single reaction protocol.

The In-Fusion method relies on the creation of primers that incorporate the mutation (base pair changes, deletions, additions) and a gene-specific priming sequence. Using an inverse PCR reaction with a circular plasmid as the template, a linearized vector is created that incorporates the mutation. The linearized vector is re-circularized via the In-Fusion reaction. High-efficiency competent cells are also provided with the kits.

Click here to learn about our In-Fusion cloning kits.

At-A-Glance Documents Images & Data Resources

Features

  • High efficiency of mutation: Perform in vitro site-directed mutagenesis with ≥ 90% efficiency
  • Versatility: The same system can generate substitutions, additions, or deletions of up to 12 bases
  • Fewer steps: One PCR reaction; no purification or in vitro digestion of PCR product; no ligation
  • Convenience: Highly competent cells provided with the kit

Applications

  • Mutagenesis
  • PCR cloning
  • HTP cloning
  • Gene design
  • Domain swapping
  • Domain modification

Choose the Right In-Fusion Cloning Plus System for Your Needs

Liquid Format

Product Name
Cat. No.
Size
Purification Kit Included
Stellar Competent Cells Included
CloneAmp HiFi PCR Premix Included
In-Fusion HD Cloning Plus
638909
10 rxns
Spin Columns
Yes
Yes
638910
50 rxns
Spin Columns
Yes
Yes
638912
100 rxns
Spin Columns
Yes
Yes
638920
96 rxns
Spin Columns
Yes
Yes
In-Fusion HD Cloning Plus CE
638916
10 rxns
Cloning Enhancer
Yes
Yes
638917
50 rxns
Cloning Enhancer
Yes
Yes
638918
100 rxns
Cloning Enhancer
Yes
Yes
638919
96rxns
Cloning Enhancer
Yes
Yes

Lyophilized Format

Product Name
Cat. No.
Size
Purification Kit Included
Stellar Competent Cells Included
CloneAmp HiFi PCR Premix Included
In-Fusion HD EcoDry Cloning Plus
638912
8 rxns
Spin Columns
Yes
Yes
638913
24 rxns
Spin Columns
Yes
Yes
638914
48 rxns
Spin Columns
Yes
Yes
638915
96 rxns
Spin Columns
Yes
Yes

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
638909 In-Fusion® HD Cloning Plus 10 Rxns $212.00 License Statements
638910 In-Fusion® HD Cloning Plus 50 Rxns $824.00 License Statements
638911 In-Fusion® HD Cloning Plus 100 Rxns $1,426.00 License Statements
638912 In-Fusion® HD EcoDry™ Cloning Plus 8 Rxns $193.00 License Statements
638913 In-Fusion® HD EcoDry™ Cloning Plus 24 Rxns $477.00 License Statements
638914 In-Fusion® HD EcoDry™ Cloning Plus 48 Rxns $942.00 License Statements
638915 In-Fusion® HD EcoDry™ Cloning Plus 96 Rxns $1,365.00 License Statements
638916 In-Fusion® HD Cloning Plus CE 10 Rxns $219.00 License Statements
638917 In-Fusion® HD Cloning Plus CE 50 Rxns $875.00 License Statements
638918 In-Fusion® HD Cloning Plus CE 100 Rxns $1,477.00 License Statements
638919 In-Fusion® HD Cloning Plus CE 96 Rxns $1,410.00 License Statements
638920 In-Fusion® HD Cloning Plus 96 Rxns $1,373.00 License Statements
 

 

Required Products
Cat. # Product Package Size Price # of Units Select
639298 CloneAmp™ HiFi PCR Premix 200 Rxns $180.00
 

 

 

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