Site-Directed Mutagenesis with In-Fusion HD Cloning System

Site-Directed Mutagenesis- PCR Based Mutagenesis with the In-Fusion HD Cloning System

In-Fusion Cloning Kits enable PCR mediated site-directed DNA mutagenesis. You can add, delete, or replace DNA segments; insert point mutations; insert restriction sites and more using a streamlined and single reaction protocol.

The In-Fusion method relies on the creation of primers that incorporate the mutation (base pair changes, deletions, additions) and a gene-specific priming sequence. Using an inverse PCR reaction with a circular plasmid as the template, a linearized vector is created that incorporates the mutation. The linearized vector is re-circularized via the In-Fusion reaction. High-efficiency competent cells are also provided with the kits. Click here to learn more.

Features

To get the best results, use CloneAmp HiFi PCR Premix with In-Fusion HD Cloning Kits.

High efficiency of mutation: Perform in vitro site-directed mutagenesis with ≥ 90% efficiency

Versatility: The same system can generate substitutions, additions, or deletions of up to 12 bases

Fewer steps: One PCR reaction; no purification or in vitro digestion of PCR product; no ligation

Convenience: Highly competent cells provided with the kit

Applications

Mutagenesis
PCR cloning
HTP cloning
Gene design
Domain swapping
Domain modification

Choose the Right In-Fusion Cloning System for Your Needs

Liquid Format

Product Name	Cat. No.	Size	Cloning Enhancer Included	Spin Columns Included	Competent Cells Included
In-Fusion HD Cloning System	639645	10 rxns		Yes	Yes
	639646	50 rxns		Yes	Yes
	639692	96 rxns		Yes	Yes
	639647	100 rxns		Yes	Yes
In-Fusion HD Cloning System CE	639636	10 rxns	Yes		Yes
	639637	50 rxns	Yes		Yes
	639693	96 rxns	Yes		Yes
	639638	100 rxns	Yes		Yes

Lyophilized Format

Product Name	Cat. No.	Size	Cloning Enhancer Included	Spin Columns Included	Competent Cells Included
In-Fusion HD EcoDry Cloning System	639684	8 rxns		Yes	Yes
	639685	24 rxns		Yes	Yes
	639686	48 rxns		Yes	Yes
	639688	96 rxns		Yes	Yes

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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