Takara's Reagent Set for Mighty Cloning Kit (Blunt End) is designed to simply and quickly prepare PCR products for cloning into blunt-ended vectors. Because the blunting and 5'-phosphorylation reactions are done simultaneously and in parallel, both blunt- and -dA ended products can be prepared in a single reaction.
This kit eliminates the need for pre-treatment (such as enzyme inactivation, removal of unused dNTPs and primers, etc.) of PCR products. In addition, the enzymes used in the blunting-kinase reaction can be removed in only 30 sections by centrifugation through a protein removal filter.*
This kit includes the same ligation reagents included in Takara's DNA Ligation Kit (Mighty Mix), which provide simple, quick and efficient ligation. The kit is suitable for blunt-end cloning of PCR products obtained with PrimeSTAR and e2TAK DNA Polymerases (only sold in the United States).
*NOTE: This kit is optimized for use with Micropure-EZ (not supplied). It can be purchased form Millipore.
See Images & Data for vector and sequence information.