Takara's DNA Ligation Kit for Long Fragments is a powerful tool for the cloning of long DNA fragments spanning 2 kb to over 10 kb in length. The kit contains an optimized ligase/buffer system that enables ligation of long fragments without the need for extensive techniques or special expertise. It is especially well suited for BAC library construction.
See accompanying product figures for a comparison of the ligation efficiencies of several commercially available ligation kits with Takara's DNA Ligation Kit for Long Fragments. Compared with other kits, Takara's cloning kit for large fragment ligation resulted in insertion efficiencies up to 50 times higher for high molecular weight DNA fragments ranging from 2 kb to 18 kb. In addition to providing superior performance regarding large fragment ligation, Takara's DNA ligation kit for long fragments also enabled fast and easy ligation of smaller size DNA inserts.