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Cloning & Competent Cells


Products >  Cloning_and_Competent_Cells >  Competent_Cells >  Electrocompetent_Cells

Electrocompetent Cells

These cells offer high transformation efficiency, making them suitable for many molecular biology applications such as generation of cDNA libraries or constructing gene banks. These cells provide alpha-complementation of the beta-galactosidase gene for blue/white screening for recombinant colonies, if desired. This E. coli strain can be used in place of DH10B cells and has several useful features: The recA1 and endA1 markers in the strain minimize recombination and degradation of plasmid DNA. In addition, alpha-complementation of the beta-galactosidase gene allows blue/white screening for positive clones. These cells can also be used for constructing genomic or cDNA libraries.

Stellar Electrocompetent Cells

Based on the HST08 E. coli strain, these cells provide high transformation efficiency, good reproducibility, and blue/ white screening when paired with pUC plasmid vectors. Stellar Electrocompetent Cells lack the gene cluster which digests foreign methylated DNA (mrr-hsdRMS-mcrBC and mcrA), and are therefore useful for cloning of methylated DNA, in addition to construction of genomic libraries and longer-length genomic libraries.

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Features

Stellar Electrocompetent Cells

Transformation Efficiency: > 1 × 109 cfu/µg

Applications

  • Cloning of large fragments
  • cDNA library construction
  • Genomic library construction
  • Long-length genomic library construction

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
636765 Stellar™ Electrocompetent Cells 10 Transformations $220.00
 

 

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