These cells offer high transformation efficiency, making them suitable for many molecular biology applications such as generation of cDNA libraries or constructing gene banks. These cells provide alpha-complementation of the beta-galactosidase gene for blue/white screening for recombinant colonies, if desired. This E. coli strain can be used in place of DH10B cells and has several useful features: The recA1 and endA1 markers in the strain minimize recombination and degradation of plasmid DNA. In addition, alpha-complementation of the beta-galactosidase gene allows blue/white screening for positive clones. These cells can also be used for constructing genomic or cDNA libraries.
Stellar Electrocompetent Cells
Based on the HST08 E. coli strain, these cells provide high transformation efficiency, good reproducibility, and blue/ white screening when paired with pUC plasmid vectors. Stellar Electrocompetent Cells lack the gene cluster which digests foreign methylated DNA (mrr-hsdRMS-mcrBC and mcrA), and are therefore useful for cloning of methylated DNA, in addition to construction of genomic libraries and longer-length genomic libraries.
