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Products >  Cloning_and_Competent_Cells >  Cloning_Vectors >  pUC_118-119_Vectors

pUC118 and pUC119 Vectors

Takara Bio's bacteriophage packaging pUC118 and pUC119 Vectors contain an intergenic region (IG region) of the M13 phage DNA, enabling their replication in the form of single-stranded DNA once transformed into bacteria with the helper phage M13K07. Single stranded pUC118 and pUC119 Vectors can then be packaged into phage particles and released from bacterial cells. Using this system, there is almost no single-stranded helper phage contamination. Furthermore, single-stranded DNA fragments of up to 7 kb can be stably obtained with no inherent deletions. pUC118 and pUC119 Vectors contain the same multiple cloning sites as pUC18 and pUC19, respectively.

  At-A-Glance   Documents   Images & Data

Features

  • Concentration: 0.5 µg/µl
  • Form: 10 mM Tris-HCI (pH8.0), 1 mM EDTA
  • Chain Length: 3,162 bp

Applications

  • Target gene cloning
  • DNA sequencing using M13 primers
  • Long DNA sequencing using Deletion Kit for Kilo-Sequencing (Cat.# 6030)
  • lac promoter gene expression 

Purity

  • >70% double-stranded, covalently closed circular form (RF1) DNA
  • Multiple cloning site sequence-verified via dideoxy DNA sequencing
  • Helper phage (M13KO7)-confirmed single-stranded DNA secretion

pUC118/119 DNA

  • It is confirmed that the vector contains a single cleavage site for EcoR I, Sac I, Kpn I, Sma I, BamH I, Xba I, Sal I, Pst I, Sph I and Hind III upon restriction analysis by the enzymes.

Storage

–20°C

GenBank

 Entry NameAccession No.
pUC118U07649U07649
pUC119U07650U07650

 
 
Products
Cat. # Product Contents Size Price Units Select
3318 pUC 118 DNA 25 ug $50.00
3319 pUC 119 DNA 25 ug $50.00
 

 

 
 

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